Hotspot

trnS-GGA-rps4

LSC · 43826-44260 · 435 bp

Back to Woodwardia

Pi 0.0544

MarkerSeek score 79.80

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 35
pi: 0.0544
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TTCGAATCCCTCCCTTTCCT	ATTGCCAATCGAGAATCCGT	50.0	45.0	58.0	57.7	530-531 bp	1.0	82.4
trnS-GGA-rps4_p2	GGTTCGAATCCCTCCCTTTC	ATTGCCAATCGAGAATCCGT	55.0	45.0	58.0	57.7	532-533 bp	1.0	82.3
trnS-GGA-rps4_p3	GTTCGAATCCCTCCCTTTCC	ATTGCCAATCGAGAATCCGT	55.0	45.0	58.0	57.7	531-532 bp	1.0	82.3
trnS-GGA-rps4_p4	TTCGAATCCCTCCCTTTCCT	GCCAATCGAGAATCCGTCAA	50.0	50.0	58.0	58.3	527-528 bp	1.0	82.3
trnS-GGA-rps4_p5	TTCGAATCCCTCCCTTTCCT	TTGCCAATCGAGAATCCGTC	50.0	50.0	58.0	58.3	529-530 bp	1.0	82.2