Hotspot

trnC-GCA-trnG-UCC

LSC · 29823-30224 · 402 bp

Back to Woodwardia

Pi 0.122

MarkerSeek score 80.80

Primer available yes

Label trnC-GCA-trnG-UCC

Feature Metadata

label_name: trnC-GCA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 67
pi: 0.122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-UCC_p1	CAACCGTTTGTCACAATCCG	AACTAAATACGTGCGCGAGT	50.0	45.0	58.0	58.0	1193-1233 bp	1.0	89.8
trnC-GCA-trnG-UCC_p2	CAACCGTTTGTCACAATCCG	ACTAAATACGTGCGCGAGTT	50.0	45.0	58.0	58.0	1192-1232 bp	1.0	89.8
trnC-GCA-trnG-UCC_p3	CGTTTGTCACAATCCGCTTC	AACTAAATACGTGCGCGAGT	50.0	45.0	58.0	58.0	1189-1229 bp	1.0	89.7
trnC-GCA-trnG-UCC_p4	CGTTTGTCACAATCCGCTTC	ACTAAATACGTGCGCGAGTT	50.0	45.0	58.0	58.0	1188-1228 bp	1.0	89.7
trnC-GCA-trnG-UCC_p5	CAACCGTTTGTCACAATCCG	ACGCCGAGGCAGAAATATTT	50.0	45.0	58.0	58.0	1316-1360 bp	1.0	89.5