Hotspot

psbM-petN

LSC · 28309-29177 · 869 bp

Back to Woodwardia

Pi 0.0622

MarkerSeek score 82.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 80
pi: 0.0622
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	ATTCAAACGGCTGCTCAGAA	TCTCACTTGTAGTTCGGGGA	45.0	50.0	58.1	57.7	922-931 bp	1.0	84.9
psbM-petN_p2	TTCAAACGGCTGCTCAGAAT	TCTCACTTGTAGTTCGGGGA	45.0	50.0	58.1	57.7	921-930 bp	1.0	84.9
psbM-petN_p3	TACATTCAAACGGCTGCTCA	TCTCACTTGTAGTTCGGGGA	45.0	50.0	57.8	57.7	925-934 bp	1.0	84.3
psbM-petN_p4	ATTCAAACGGCTGCTCAGAA	TTGTAGTTCGGGGAAGAAGC	45.0	50.0	58.1	57.5	916-925 bp	1.0	83.6
psbM-petN_p5	TTCAAACGGCTGCTCAGAAT	TTGTAGTTCGGGGAAGAAGC	45.0	50.0	58.1	57.5	915-924 bp	1.0	83.6