Hotspot

matK-rps16

LSC · 2944-3564 · 621 bp

Back to Woodwardia

Pi 0.0683
MarkerSeek score 80.00
Primer available yes
Label matK-rps16

Feature Metadata

label_name
matK-rps16
feature_type
igs
region
LSC
length_bp
621
variable_sites
63
pi
0.0683
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-rps16_p1 TCGTGTCCATGAAGCTAACG ATCCCCTCGCTACTATTGCT 50.0 50.0 58.0 58.0 1439 bp 0.333333 53.1
matK-rps16_p2 GGCGAACCAGAGTTTCTAGG ATCCCCTCGCTACTATTGCT 55.0 50.0 58.0 58.0 1245 bp 0.333333 53.1
matK-rps16_p3 TGCTAAGTCCTTCGCTGTTC ATCCCCTCGCTACTATTGCT 50.0 50.0 57.9 58.0 813 bp 0.333333 52.6
matK-rps16_p4 TCGTGTCCATGAAGCTAACG AGTGAAAAGAAGGAAGGCGG 50.0 50.0 58.0 58.1 1822 bp 0.333333 52.4
matK-rps16_p5 TACTTCTTCGCCCTTCTTGC ATCCCCTCGCTACTATTGCT 50.0 50.0 57.9 58.0 1371 bp 0.333333 52.4