primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	matK-rps16	1	TCGTGTCCATGAAGCTAACG	ATCCCCTCGCTACTATTGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026106	2944	3564	1439-1439	0.333333	53.1
matK-rps16_p2	matK-rps16	2	GGCGAACCAGAGTTTCTAGG	ATCCCCTCGCTACTATTGCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.028445	2944	3564	1245-1245	0.333333	53.1
matK-rps16_p3	matK-rps16	3	TGCTAAGTCCTTCGCTGTTC	ATCCCCTCGCTACTATTGCT	20	20	50.0	50.0	57.9	58.0	5.3	0.0	0.097191	2944	3564	813-813	0.333333	52.6
matK-rps16_p4	matK-rps16	4	TCGTGTCCATGAAGCTAACG	AGTGAAAAGAAGGAAGGCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.111641	2944	3564	1822-1822	0.333333	52.4
matK-rps16_p5	matK-rps16	5	TACTTCTTCGCCCTTCTTGC	ATCCCCTCGCTACTATTGCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.111667	2944	3564	1371-1371	0.333333	52.4
atpH-atpI_p1	atpH-atpI	1	AGATAGATGCGGCAGGTACT	GTTGGTAGTAGCTGTTCCCG	20	20	50.0	55.0	58.0	58.0	9.3	0.0	0.008476	13519	14271	1286-1294	1.000000	86.8
atpH-atpI_p2	atpH-atpI	2	AGATAGATGCGGCAGGTACT	GTACCCATGACGCCTTTAGG	20	20	50.0	55.0	58.0	58.1	9.3	0.0	0.053040	13519	14271	1240-1248	1.000000	86.5
atpH-atpI_p3	atpH-atpI	3	AGATAGATGCGGCAGGTACT	TCTACCAAACCCCCATCACT	20	20	50.0	50.0	58.0	57.9	9.3	0.0	0.056252	13519	14271	1342-1350	1.000000	86.2
atpH-atpI_p4	atpH-atpI	4	AGATAGATGCGGCAGGTACT	GGCGAATCGTTCAGTTACCT	20	20	50.0	50.0	58.0	58.0	9.3	4.1	0.008476	13519	14271	1530-1538	0.666667	71.6
atpH-atpI_p5	atpH-atpI	5	AGATAGATGCGGCAGGTACT	ATCAACACTACTGTCGCGTC	20	20	50.0	50.0	58.0	58.0	9.3	5.8	0.023964	13519	14271	1488-1488	0.333333	53.1
rpoB-trnD-GUC_p1	rpoB-trnD-GUC	1	CAAGTTCTGGTAGTACGGGC	GCGGTGCTCTAACCAATTGA	20	20	55.0	50.0	58.0	58.6	0.0	7.1	0.556986	26519	27309	829-911	1.000000	83.4
rpoB-trnD-GUC_p2	rpoB-trnD-GUC	2	GCAAGTTCTGGTAGTACGGG	GCGGTGCTCTAACCAATTGA	20	20	55.0	50.0	58.0	58.6	0.0	7.1	0.556986	26519	27309	830-912	1.000000	83.4
rpoB-trnD-GUC_p3	rpoB-trnD-GUC	3	AGTTCTGGTAGTACGGGCAT	GCGGTGCTCTAACCAATTGA	20	20	50.0	50.0	58.1	58.6	0.0	7.1	0.689034	26519	27309	827-909	1.000000	82.4
rpoB-trnD-GUC_p4	rpoB-trnD-GUC	4	AAGTTCTGGTAGTACGGGCA	GCGGTGCTCTAACCAATTGA	20	20	50.0	50.0	58.4	58.6	0.0	7.1	0.914867	26519	27309	828-910	1.000000	80.6
rpoB-trnD-GUC_p5	rpoB-trnD-GUC	5	CAAGTTCTGGTAGTACGGGC	GGATTGACGGGTCTCGAAC	20	19	55.0	57.9	58.0	58.0	0.0	0.0	1.052250	26519	27309	870-952	1.000000	79.1
psbM-petN_p1	psbM-petN	1	ATTCAAACGGCTGCTCAGAA	TCTCACTTGTAGTTCGGGGA	20	20	45.0	50.0	58.1	57.7	0.0	0.0	0.401479	28309	29177	922-931	1.000000	84.9
psbM-petN_p2	psbM-petN	2	TTCAAACGGCTGCTCAGAAT	TCTCACTTGTAGTTCGGGGA	20	20	45.0	50.0	58.1	57.7	0.0	0.0	0.401479	28309	29177	921-930	1.000000	84.9
psbM-petN_p3	psbM-petN	3	TACATTCAAACGGCTGCTCA	TCTCACTTGTAGTTCGGGGA	20	20	45.0	50.0	57.8	57.7	0.0	0.0	0.475092	28309	29177	925-934	1.000000	84.3
psbM-petN_p4	psbM-petN	4	ATTCAAACGGCTGCTCAGAA	TTGTAGTTCGGGGAAGAAGC	20	20	45.0	50.0	58.1	57.5	0.0	0.0	0.571346	28309	29177	916-925	1.000000	83.6
psbM-petN_p5	psbM-petN	5	TTCAAACGGCTGCTCAGAAT	TTGTAGTTCGGGGAAGAAGC	20	20	45.0	50.0	58.1	57.5	0.0	0.0	0.571346	28309	29177	915-924	1.000000	83.6
petN-trnC-GCA_p1	petN-trnC-GCA	1	GCCCAAGCTATAGTAACTAGATCC	AAGACGGAGGACTGCAAATC	24	20	45.8	50.0	58.0	57.9	0.0	0.0	4.132350	29268	29750	557-565	1.000000	55.3
petN-trnC-GCA_p2	petN-trnC-GCA	2	GCCCAAGCTATAGTAACTAGATCC	CCAGTTCGAATCTGGGTGTC	24	20	45.8	55.0	58.0	58.3	0.0	26.3	4.304892	29268	29750	528-536	1.000000	54.4
petN-trnC-GCA_p3	petN-trnC-GCA	3	CCCAAGCTATAGTAACTAGATCCG	AAGACGGAGGACTGCAAATC	24	20	45.8	50.0	57.8	57.9	0.0	0.0	4.297968	29268	29750	556-564	1.000000	54.0
petN-trnC-GCA_p4	petN-trnC-GCA	4	GCCCAAGCTATAGTAACTAGATCC	TCAAGTGGTAAGACGGAGGA	24	20	45.8	50.0	58.0	57.7	0.0	0.0	4.329444	29268	29750	566-574	1.000000	53.6
petN-trnC-GCA_p5	petN-trnC-GCA	5	GCCCAAGCTATAGTAACTAGATCC	AAGTGGTAAGACGGAGGACT	24	20	45.8	50.0	58.0	57.7	0.0	0.0	4.337603	29268	29750	564-572	1.000000	53.6
trnC-GCA-trnG-UCC_p1	trnC-GCA-trnG-UCC	1	CAACCGTTTGTCACAATCCG	AACTAAATACGTGCGCGAGT	20	20	50.0	45.0	58.0	58.0	0.0	28.7	0.044339	29823	30224	1193-1233	1.000000	89.8
trnC-GCA-trnG-UCC_p2	trnC-GCA-trnG-UCC	2	CAACCGTTTGTCACAATCCG	ACTAAATACGTGCGCGAGTT	20	20	50.0	45.0	58.0	58.0	0.0	28.7	0.044339	29823	30224	1192-1232	1.000000	89.8
trnC-GCA-trnG-UCC_p3	trnC-GCA-trnG-UCC	3	CGTTTGTCACAATCCGCTTC	AACTAAATACGTGCGCGAGT	20	20	50.0	45.0	58.0	58.0	0.0	28.7	0.062830	29823	30224	1189-1229	1.000000	89.7
trnC-GCA-trnG-UCC_p4	trnC-GCA-trnG-UCC	4	CGTTTGTCACAATCCGCTTC	ACTAAATACGTGCGCGAGTT	20	20	50.0	45.0	58.0	58.0	0.0	28.7	0.062830	29823	30224	1188-1228	1.000000	89.7
trnC-GCA-trnG-UCC_p5	trnC-GCA-trnG-UCC	5	CAACCGTTTGTCACAATCCG	ACGCCGAGGCAGAAATATTT	20	20	50.0	45.0	58.0	58.0	0.0	15.0	0.069424	29823	30224	1316-1360	1.000000	89.5
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	TTCGAATCCCTCCCTTTCCT	ATTGCCAATCGAGAATCCGT	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.365218	43826	44260	530-531	1.000000	82.4
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	GGTTCGAATCCCTCCCTTTC	ATTGCCAATCGAGAATCCGT	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.371881	43826	44260	532-533	1.000000	82.3
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GTTCGAATCCCTCCCTTTCC	ATTGCCAATCGAGAATCCGT	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.371881	43826	44260	531-532	1.000000	82.3
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	TTCGAATCCCTCCCTTTCCT	GCCAATCGAGAATCCGTCAA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.381168	43826	44260	527-528	1.000000	82.3
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	TTCGAATCCCTCCCTTTCCT	TTGCCAATCGAGAATCCGTC	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.381168	43826	44260	529-530	1.000000	82.2
rps4-trnL-UAA_p1	rps4-trnL-UAA	1	TCACTTTCAAACGAGGTCCTC	ATTGCCCGATAAATGGTCCC	21	20	47.6	50.0	57.9	58.0	9.1	0.0	1.112266	44882	45537	790-812	1.000000	77.7
rps4-trnL-UAA_p2	rps4-trnL-UAA	2	TCACTTTCAAACGAGGTCCTC	CTGCCACTTGATACGTCTCC	21	20	47.6	55.0	57.9	58.1	9.1	0.0	1.179747	44882	45537	749-771	1.000000	77.5
rps4-trnL-UAA_p3	rps4-trnL-UAA	3	TCACTTTCAAACGAGGTCCTC	CTCAATCTAGCTGAGTCCGC	21	20	47.6	55.0	57.9	57.9	9.1	11.6	1.240382	44882	45537	720-742	1.000000	77.3
rps4-trnL-UAA_p4	rps4-trnL-UAA	4	TCACTTTCAAACGAGGTCCTC	GCTCAATCTAGCTGAGTCCG	21	20	47.6	55.0	57.9	57.9	9.1	17.8	1.240382	44882	45537	721-743	1.000000	77.3
rps4-trnL-UAA_p5	rps4-trnL-UAA	5	TCACTTTCAAACGAGGTCCTC	CAATCTAGCTGAGTCCGCTG	21	20	47.6	55.0	57.9	58.1	9.1	18.3	1.257265	44882	45537	718-740	1.000000	77.2
trnV-UAC_p1	trnV-UAC	1	AGATACGGTTTCAACGTCGG	GCGATACTCTAACCGCTGAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.052629	48854	49472	1430-1448	1.000000	86.3
trnV-UAC_p2	trnV-UAC	2	ATACGGTTTCAACGTCGGAG	GCGATACTCTAACCGCTGAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.052629	48854	49472	1428-1446	1.000000	86.3
trnV-UAC_p3	trnV-UAC	3	AGATACGGTTTCAACGTCGG	TACGTCATGATGGGCAATGG	20	20	50.0	50.0	58.0	58.0	0.0	19.0	0.047367	48854	49472	1867-1891	1.000000	85.3
trnV-UAC_p4	trnV-UAC	4	ATACGGTTTCAACGTCGGAG	TACGTCATGATGGGCAATGG	20	20	50.0	50.0	58.0	58.0	0.0	19.0	0.047367	48854	49472	1865-1889	1.000000	85.2
trnV-UAC_p5	trnV-UAC	5	GATACGGTTTCAACGTCGGA	TACGTCATGATGGGCAATGG	20	20	50.0	50.0	58.0	58.0	0.0	19.0	0.052812	48854	49472	1866-1890	1.000000	85.2
petA-psbJ_p1	petA-psbJ	1	TGTTGGTGGATTTGGTCAGG	TCGTGGTTGGTAGGTACTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006396	59657	60488	1094-1122	1.000000	87.1
petA-psbJ_p2	petA-psbJ	2	GTATCCAGGGTCTCGTAGCT	TCGTGGTTGGTAGGTACTGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016286	59657	60488	1041-1069	1.000000	87.1
petA-psbJ_p3	petA-psbJ	3	GGGCAAATCTACCCAGATGG	TCGTGGTTGGTAGGTACTGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.013469	59657	60488	1324-1352	1.000000	86.6
petA-psbJ_p4	petA-psbJ	4	TAATCGCATTCCAGCCGAAA	TCGTGGTTGGTAGGTACTGT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.031610	59657	60488	1535-1563	1.000000	86.0
petA-psbJ_p5	petA-psbJ	5	ATCGTTTCAGAGTTACCGCC	TCGTGGTTGGTAGGTACTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006322	59657	60488	1493-1493	0.333333	53.3
trnR-UCU-rps12_p1	trnR-UCU-rps12	1	AGCCAGGATCAAACTCTCCA	GAACTCTCGCGACTTTACGT	20	20	50.0	50.0	58.3	58.0	0.0	3.7	0.377458	92937	95859	3000-3000	0.333333	50.3
trnR-UCU-rps12_p2	trnR-UCU-rps12	2	GAGCCAGGATCAAACTCTCC	CTCTCGCGACTTTACGTAGG	20	20	55.0	55.0	57.7	57.9	0.0	3.7	0.412307	92937	95859	2998-2998	0.333333	50.0
trnR-UCU-rps12_p3	trnR-UCU-rps12	3	AGCCAGGATCAAACTCTCCA	CTCTCGCGACTTTACGTAGG	20	20	50.0	55.0	58.3	57.9	0.0	3.7	0.426875	92937	95859	2997-2997	0.333333	49.9
trnR-UCU-rps12_p4	trnR-UCU-rps12	4	GAGCCAGGATCAAACTCTCC	CGACTTTACGTAGGGCAGAG	20	20	55.0	55.0	57.7	57.8	0.0	0.0	0.514293	92937	95859	2992-2992	0.333333	49.2
trnR-UCU-rps12_p5	trnR-UCU-rps12	5	AGCCAGGATCAAACTCTCCA	CGACTTTACGTAGGGCAGAG	20	20	50.0	55.0	58.3	57.8	0.0	0.0	0.528862	92937	95859	2991-2991	0.333333	49.1
rpl21-rpl32_p1	rpl21-rpl32	1	ATGCAGTTGTCAAAGGCAGA	GAGGTCACAGGTTCGAATCC	20	20	45.0	55.0	58.0	58.0	15.2	4.4	0.026067	109945	110124	1086-1094	1.000000	89.6
rpl21-rpl32_p2	rpl21-rpl32	2	ATGCAGTTGTCAAAGGCAGA	GGAGGTCACAGGTTCGAATC	20	20	45.0	55.0	58.0	58.0	15.2	0.0	0.026067	109945	110124	1087-1095	1.000000	89.6
rpl21-rpl32_p3	rpl21-rpl32	3	ATGCAGTTGTCAAAGGCAGA	AGGAAAAAGCCCTTAACGCA	20	20	45.0	45.0	58.0	58.0	15.2	0.0	0.024821	109945	110124	437-450	1.000000	87.5
rpl21-rpl32_p4	rpl21-rpl32	4	CAATAATTGACATCGGCGGC	AGGAAAAAGCCCTTAACGCA	20	20	50.0	45.0	58.0	58.0	7.2	0.0	0.046850	109945	110124	611-624	1.000000	86.7
rpl21-rpl32_p5	rpl21-rpl32	5	GCAATAATTGACATCGGCGG	AGGAAAAAGCCCTTAACGCA	20	20	50.0	45.0	58.0	58.0	7.2	0.0	0.046850	109945	110124	612-625	1.000000	86.7
rpl32-trnP-GGG_p1	rpl32-trnP-GGG	1	ATGCAGTTGTCAAAGGCAGA	GAGGTCACAGGTTCGAATCC	20	20	45.0	55.0	58.0	58.0	15.2	4.4	0.026067	110293	110829	1086-1094	1.000000	89.6
rpl32-trnP-GGG_p2	rpl32-trnP-GGG	2	ATGCAGTTGTCAAAGGCAGA	GGAGGTCACAGGTTCGAATC	20	20	45.0	55.0	58.0	58.0	15.2	0.0	0.026067	110293	110829	1087-1095	1.000000	89.6
rpl32-trnP-GGG_p3	rpl32-trnP-GGG	3	TGCGTTAAGGGCTTTTTCCT	CTGGCACTGCTTCCTAAGAG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.031649	110293	110829	1008-1018	1.000000	89.6
rpl32-trnP-GGG_p4	rpl32-trnP-GGG	4	TGCGTTAAGGGCTTTTTCCT	GAGGTCACAGGTTCGAATCC	20	20	45.0	55.0	58.0	58.0	0.0	4.4	0.021834	110293	110829	656-677	1.000000	89.1
rpl32-trnP-GGG_p5	rpl32-trnP-GGG	5	TGCGTTAAGGGCTTTTTCCT	GGAGGTCACAGGTTCGAATC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.021834	110293	110829	657-678	1.000000	89.1
ccsA-ndhD_p1	ccsA-ndhD	1	TGCAGTCTCATCGGTCAATG	ATTTTGGTCCGAGGGAGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053203	112483	112895	1088-1107	1.000000	87.3
ccsA-ndhD_p2	ccsA-ndhD	2	TGCAGTCTCATCGGTCAATG	TTTGGTCCGAGGGAGGTATT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053203	112483	112895	1086-1105	1.000000	87.3
ccsA-ndhD_p3	ccsA-ndhD	3	TGCAGTCTCATCGGTCAATG	TTTTGGTCCGAGGGAGGTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053203	112483	112895	1087-1106	1.000000	87.3
ccsA-ndhD_p4	ccsA-ndhD	4	TGCAGTCTCATCGGTCAATG	AGCGTTATTCTCCCTTGCAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044174	112483	112895	1426-1445	1.000000	86.4
ccsA-ndhD_p5	ccsA-ndhD	5	GTTTGCCTCTTGGGAGTCAA	AGCGTTATTCTCCCTTGCAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048582	112483	112895	942-954	0.666667	72.1
rps12-ICG91_pgr003_p1	rps12-ICG91_pgr003	1	GAACTCTCGCGACTTTACGT	AGCCAGGATCAAACTCTCCA	20	20	50.0	50.0	58.0	58.3	3.7	0.0	0.377458	139846	142768	3000-3000	0.333333	50.3
rps12-ICG91_pgr003_p2	rps12-ICG91_pgr003	2	CTCTCGCGACTTTACGTAGG	GAGCCAGGATCAAACTCTCC	20	20	55.0	55.0	57.9	57.7	3.7	0.0	0.412307	139846	142768	2998-2998	0.333333	50.0
rps12-ICG91_pgr003_p3	rps12-ICG91_pgr003	3	CTCTCGCGACTTTACGTAGG	AGCCAGGATCAAACTCTCCA	20	20	55.0	50.0	57.9	58.3	3.7	0.0	0.426875	139846	142768	2997-2997	0.333333	49.9
rps12-ICG91_pgr003_p4	rps12-ICG91_pgr003	4	CGACTTTACGTAGGGCAGAG	GAGCCAGGATCAAACTCTCC	20	20	55.0	55.0	57.8	57.7	0.0	0.0	0.514293	139846	142768	2992-2992	0.333333	49.2
rps12-ICG91_pgr003_p5	rps12-ICG91_pgr003	5	CGACTTTACGTAGGGCAGAG	AGCCAGGATCAAACTCTCCA	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.528862	139846	142768	2991-2991	0.333333	49.1