Hotspot

ycf1

Genome · 119774-124879 · 5106 bp

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Pi 0.0446

MarkerSeek score 64.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5106
variable_sites: 227
pi: 0.0446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTAGCCACGGCATCAAGTC	ACTTTCTATCCGACAAGATGCT	57.9	40.9	58.3	57.3	164 bp	1.0	54.3
ycf1_p2	CTAGCCACGGCATCAAGTC	AACTTTCTATCCGACAAGATGCT	57.9	39.1	58.3	57.9	165 bp	1.0	51.5
ycf1_p3	TAGCCACGGCATCAAGTCAA	ACTTTCTATCCGACAAGATGCT	50.0	40.9	59.7	57.3	163 bp	1.0	51.5
ycf1_p4	CTAGCCACGGCATCAAGTC	ACTTTCTATCCGACAAGATGC	57.9	42.9	58.3	55.8	164 bp	1.0	50.6
ycf1_p5	CTAGCCACGGCATCAAGTC	CGTACGAAATGGGGATATACAGA	57.9	43.5	58.3	57.6	188 bp	1.0	48.4