Hotspot

ndhF

Genome · 105884-108112 · 2229 bp

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Pi 0.0592

MarkerSeek score 76.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2229
variable_sites: 132
pi: 0.0592
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAATTGGAGCTTCGTGAAAC	TGACTCCTAATCAAAAGAAGTTGA	47.6	33.3	58.2	56.0	2316 bp	1.0	44.6
ndhF_p2	CGAATTGGAGCTTCGTGAAA	TGACTCCTAATCAAAAGAAGTTGA	45.0	33.3	56.7	56.0	2316 bp	1.0	44.6
ndhF_p3	CGAATTGGAGCTTCGTGAAAC	TGACTCCTAATCAAAAGAAGTTGAA	47.6	32.0	58.2	56.6	2316 bp	1.0	44.6
ndhF_p4	CGAATTGGAGCTTCGTGAAAC	TTGACTCCTAATCAAAAGAAGTTGA	47.6	32.0	58.2	56.6	2317 bp	1.0	44.6
ndhF_p5	CGAATTGGAGCTTCGTGAAAC	TGACTCCTAATCAAAAGAAGTTG	47.6	34.8	58.2	54.6	2316 bp	1.0	44.6