Hotspot

matK

Genome · 1444-2946 · 1503 bp

Back to Woodsia

Pi 0.0459

MarkerSeek score 70.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: Genome
length_bp: 1503
variable_sites: 69
pi: 0.0459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	GTGACCAAATCATTTGCCCG	GAACAACCTCTCTTGCCCAA	50.0	50.0	58.0	58.0	2521-2527 bp	1.0	83.6
matK_p2	GTGACCAAATCATTTGCCCG	TCGGAGGGACTACTGGTTTT	50.0	50.0	58.0	58.0	2657-2663 bp	1.0	83.6
matK_p3	GTGACCAAATCATTTGCCCG	TTCGGAGGGACTACTGGTTT	50.0	50.0	58.0	58.0	2658-2664 bp	1.0	83.6
matK_p4	GTGACCAAATCATTTGCCCG	TTTCGGAGGGACTACTGGTT	50.0	50.0	58.0	58.0	2659-2665 bp	1.0	83.6
matK_p5	GTGACCAAATCATTTGCCCG	CTAATGGGCCCAGCTTACAG	50.0	55.0	58.0	58.0	2767-2773 bp	1.0	83.4