Hotspot

trnP-GGG-trnL-UAG

Genome · 109847-110112 · 266 bp

Back to Woodsia

Pi 0.125

MarkerSeek score 82.60

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: Genome
length_bp: 266
variable_sites: 31
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GATTCGAACCTGTGACCTCC	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.0	58.0	364-381 bp	1.0	90.3
trnP-GGG-trnL-UAG_p2	GATTCGAACCTGTGACCTCC	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.0	58.0	362-379 bp	1.0	90.3
trnP-GGG-trnL-UAG_p3	GGATTCGAACCTGTGACCTC	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.0	58.0	363-380 bp	1.0	90.3
trnP-GGG-trnL-UAG_p4	GGATTCGAACCTGTGACCTC	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.0	58.0	365-382 bp	1.0	90.2
trnP-GGG-trnL-UAG_p5	GATTCGAACCTGTGACCTCC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.0	57.9	351-368 bp	1.0	89.8