Hotspot

rpl32-trnP-GGG

Genome · 109127-109772 · 646 bp

Back to Woodsia

Pi 0.0748

MarkerSeek score 77.60

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 646
variable_sites: 48
pi: 0.0748
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	CGGCCGTTCAAGAAGTTTTC	GAGGTCACAGGTTCGAATCC	50.0	55.0	58.0	58.0	735-738 bp	1.0	85.6
rpl32-trnP-GGG_p2	CGGCCGTTCAAGAAGTTTTC	GGAGGTCACAGGTTCGAATC	50.0	55.0	58.0	58.0	736-739 bp	1.0	85.6
rpl32-trnP-GGG_p3	TTTAACCGGCCGTTCAAGAA	GAGGTCACAGGTTCGAATCC	45.0	55.0	58.0	58.0	741-744 bp	1.0	85.6
rpl32-trnP-GGG_p4	CGGTTGCCTCGAAGAAAAAC	GAGGTCACAGGTTCGAATCC	50.0	55.0	58.0	58.0	1173-1176 bp	1.0	84.3
rpl32-trnP-GGG_p5	CGGTTGCCTCGAAGAAAAAC	GGAGGTCACAGGTTCGAATC	50.0	55.0	58.0	58.0	1174-1177 bp	1.0	84.3