Hotspot

trnI-CAU-ndhB

Genome · 82397-82848 · 452 bp

Back to Woodsia

Pi 0.0318

MarkerSeek score 62.50

Primer available yes

Label trnI-CAU-ndhB

Feature Metadata

label_name: trnI-CAU-ndhB
feature_type: igs
region: Genome
length_bp: 452
variable_sites: 14
pi: 0.0318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ndhB_p1	CGCTTTGTCCGTTTTCCTTC	AAAACCGGATAGTGGAGGGA	50.0	50.0	58.0	58.0	1144-1155 bp	1.0	82.8
trnI-CAU-ndhB_p2	GCTTTGTCCGTTTTCCTTCG	AAAACCGGATAGTGGAGGGA	50.0	50.0	58.0	58.0	1143-1154 bp	1.0	82.8
trnI-CAU-ndhB_p3	TGAACCTGTGAATTCGCCAA	AAAACCGGATAGTGGAGGGA	45.0	50.0	58.0	58.0	963-975 bp	1.0	82.0
trnI-CAU-ndhB_p4	TTGAACCTGTGAATTCGCCA	AAAACCGGATAGTGGAGGGA	45.0	50.0	58.0	58.0	964-976 bp	1.0	82.0
trnI-CAU-ndhB_p5	TGAACCTGTGAATTCGCCAA	AGCTTTCCATCCATGCCAAA	45.0	45.0	58.0	58.1	1002-1014 bp	1.0	81.7