Hotspot

trnW-CCA-trnP-UGG

Genome · 62635-62803 · 169 bp

Back to Woodsia

Pi 0.0798

MarkerSeek score 79.00

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: Genome
length_bp: 169
variable_sites: 13
pi: 0.0798
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	ACCTACGACATCGGGTTTTG	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.9	57.9	275-280 bp	1.0	83.7
trnW-CCA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	CGGTAGCGTGTTTGTTTTGG	50.0	50.0	57.9	58.3	276-281 bp	1.0	82.7
trnW-CCA-trnP-UGG_p3	CTACGACATCGGGTTTTGGA	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.6	57.9	273-278 bp	1.0	81.5
trnW-CCA-trnP-UGG_p4	TACGACATCGGGTTTTGGAG	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.6	57.9	272-277 bp	1.0	81.5
trnW-CCA-trnP-UGG_p5	GAACCTACGACATCGGGTTT	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.6	57.9	277-282 bp	1.0	81.4