Hotspot

trnS-GGA-rps4

Genome · 43551-44019 · 469 bp

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Pi 0.0736

MarkerSeek score 76.70

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: Genome
length_bp: 469
variable_sites: 34
pi: 0.0736
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TTCGAATCCCTCCCTTTCCT	TGAGTATTACTCCCGAAAAGCT	50.0	40.9	58.0	56.9	507-514 bp	1.0	61.5
trnS-GGA-rps4_p2	GGTTCGAATCCCTCCCTTTC	TGAGTATTACTCCCGAAAAGCT	55.0	40.9	58.0	56.9	509-516 bp	1.0	61.4
trnS-GGA-rps4_p3	GTTCGAATCCCTCCCTTTCC	TGAGTATTACTCCCGAAAAGCT	55.0	40.9	58.0	56.9	508-515 bp	1.0	61.4
trnS-GGA-rps4_p4	TTCGAATCCCTCCCTTTCCT	ATTACTCCCGAAAAGCTTGA	50.0	40.0	58.0	54.7	502-509 bp	1.0	59.5
trnS-GGA-rps4_p5	GTTCGAATCCCTCCCTTTCC	ATTACTCCCGAAAAGCTTGA	55.0	40.0	58.0	54.7	503-510 bp	1.0	59.5