Hotspot

psbM-petN

Genome · 28230-29091 · 862 bp

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Pi 0.0629

MarkerSeek score 75.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 862
variable_sites: 54
pi: 0.0629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAGGAAGTAACGGCGGAATC	CTTGCTACCGAACATTCCGA	50.0	50.0	58.0	58.0	2046 bp	0.5	59.8
psbM-petN_p2	AAGGAAGTAACGGCGGAATC	TGCATACGTCACTTGCTACC	50.0	50.0	58.0	58.0	2057 bp	0.5	59.8
psbM-petN_p3	AAGGAAGTAACGGCGGAATC	GTCCTCGGATTAGCGTTGTT	50.0	50.0	58.0	58.0	2002 bp	0.5	59.8
psbM-petN_p4	AAGGAAGTAACGGCGGAATC	CGACAACGCGTTTTAAGCTT	50.0	45.0	58.0	58.0	1536 bp	0.5	59.7
psbM-petN_p5	AAGGAAGTAACGGCGGAATC	TTTTCGTCCTCGGATTAGCG	50.0	50.0	58.0	58.1	2007 bp	0.5	59.2