Hotspot

trnY-GUA-trnE-UUC

Genome · 27469-27615 · 147 bp

Back to Woodsia

Pi 0.0986

MarkerSeek score 81.90

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: Genome
length_bp: 147
variable_sites: 14
pi: 0.0986
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	GTCCCCATTAACCACTCGAG	AAAGTAATCAGGCCCCCATC	55.0	50.0	57.7	57.2	260-264 bp	1.0	77.3
trnY-GUA-trnE-UUC_p2	CCCATTAACCACTCGAGCAT	AAAGTAATCAGGCCCCCATC	50.0	50.0	57.7	57.2	257-261 bp	1.0	77.2
trnY-GUA-trnE-UUC_p3	CTGGATTCGAACCAGCGTAG	AAAGTAATCAGGCCCCCATC	55.0	50.0	58.4	57.2	304-308 bp	1.0	75.4
trnY-GUA-trnE-UUC_p4	GTCCCCATTAACCACTCGAG	AAAAAGTAATCAGGCCCCCA	55.0	45.0	57.7	57.0	262-266 bp	1.0	75.2
trnY-GUA-trnE-UUC_p5	TTGCCAGCGGATTTACAGTC	AAAGTAATCAGGCCCCCATC	50.0	50.0	58.6	57.2	281-285 bp	1.0	74.9