Hotspot

trnD-GUC-trnY-GUA

Genome · 27335-27386 · 52 bp

Back to Woodsia

Pi 0.0769
MarkerSeek score 76.70
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
Genome
length_bp
52
variable_sites
4
pi
0.0769
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 TCAATTGGTTAGAGCACCGC CTCGAGTGGTTAATGGGGAC 50.0 55.0 58.6 57.7 188 bp 1.0 74.8
trnD-GUC-trnY-GUA_p2 TCAATTGGTTAGAGCACCGC ATGCTCGAGTGGTTAATGGG 50.0 50.0 58.6 57.7 191 bp 1.0 74.6
trnD-GUC-trnY-GUA_p3 TCAATTGGTTAGAGCACCGC GACTGTAAATCCGCTGGCAA 50.0 50.0 58.6 58.6 167 bp 1.0 73.1
trnD-GUC-trnY-GUA_p4 GGTTCGAGACCCGTCAATC CTCGAGTGGTTAATGGGGAC 57.9 55.0 58.0 57.7 147 bp 1.0 71.3
trnD-GUC-trnY-GUA_p5 GTTCGAGACCCGTCAATCC CTCGAGTGGTTAATGGGGAC 57.9 55.0 58.0 57.7 146 bp 1.0 71.3