Hotspot

rpoB-trnD-GUC

Genome · 26468-27260 · 793 bp

Back to Woodsia

Pi 0.0508

MarkerSeek score 69.90

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 793
variable_sites: 27
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAGTACGGGC	TTATCGGGATTGACGGGTCT	55.0	50.0	58.0	58.2	635-893 bp	1.0	85.1
rpoB-trnD-GUC_p2	GCAAGTTCTGGTAGTACGGG	TTATCGGGATTGACGGGTCT	55.0	50.0	58.0	58.2	636-894 bp	1.0	85.1
rpoB-trnD-GUC_p3	CAAGTTCTGGTAGTACGGGC	GTTTATCGGGATTGACGGGT	55.0	50.0	58.0	57.7	637-895 bp	1.0	84.2
rpoB-trnD-GUC_p4	GCAAGTTCTGGTAGTACGGG	GTTTATCGGGATTGACGGGT	55.0	50.0	58.0	57.7	638-896 bp	1.0	84.2
rpoB-trnD-GUC_p5	AGTTCTGGTAGTACGGGCAT	TTATCGGGATTGACGGGTCT	50.0	50.0	58.1	58.2	633-891 bp	1.0	84.0