Hotspot

atpH-atpI

Genome · 13537-14243 · 707 bp

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Pi 0.0509

MarkerSeek score 75.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 707
variable_sites: 36
pi: 0.0509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCAGCAGAAATCAATGGGT	CTGCAGCTCATATTGGGGAA	45.0	50.0	57.8	57.6	772-786 bp	1.0	79.1
atpH-atpI_p2	CTAACCCAGCGGCAATAACA	CTGCAGCTCATATTGGGGAA	50.0	50.0	58.3	57.6	794-808 bp	1.0	78.7
atpH-atpI_p3	TAACCCAGCGGCAATAACAG	CTGCAGCTCATATTGGGGAA	50.0	50.0	58.3	57.6	793-807 bp	1.0	78.7
atpH-atpI_p4	GGCAATAACAGAAGCAGCAG	CTGCAGCTCATATTGGGGAA	50.0	50.0	57.4	57.6	784-798 bp	1.0	76.4
atpH-atpI_p5	AGCGGCAATAACAGAAGCAG	CTGCAGCTCATATTGGGGAA	50.0	50.0	58.9	57.6	787-801 bp	1.0	73.5