Hotspot

trnS-GCU-ycf12

Genome · 7955-8522 · 568 bp

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Pi 0.0675
MarkerSeek score 78.60
Primer available yes
Label trnS-GCU-ycf12

Feature Metadata

label_name
trnS-GCU-ycf12
feature_type
igs
region
Genome
length_bp
568
variable_sites
38
pi
0.0675
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-ycf12_p1 AGCAATCCGACGCTTTAGAC CTAAGGGTCCTGATGCAACC 50.0 55.0 58.4 58.0 669-671 bp 1.0 81.9
trnS-GCU-ycf12_p2 CAATCCGACGCTTTAGACCC CTAAGGGTCCTGATGCAACC 55.0 55.0 58.7 58.0 667-669 bp 1.0 79.1
trnS-GCU-ycf12_p3 AGCAATCCGACGCTTTAGAC TAAGGGTCCTGATGCAACCA 50.0 50.0 58.4 58.6 668-670 bp 1.0 77.2
trnS-GCU-ycf12_p4 AGCAATCCGACGCTTTAGAC AACCACCAACGCCAAAGTAG 50.0 50.0 58.4 58.7 653-655 bp 1.0 76.9
trnS-GCU-ycf12_p5 AGCAATCCGACGCTTTAGAC AACGCCAAAGTAGCAAGTTG 50.0 45.0 58.4 57.2 646-648 bp 1.0 76.3