Hotspot

rps16

LSC · 4737-6001 · 1265 bp

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Pi 0.0783

MarkerSeek score 84.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1265
variable_sites: 167
pi: 0.0783
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCTTTCAGGATCAGTCGTGG	TGGTTTTCTTGCCGATCCTT	55.0	45.0	58.0	57.7	1972-1981 bp	0.5	60.3
rps16_p2	CCTTTCAGGATCAGTCGTGG	TTGGTTTTCTTGCCGATCCT	55.0	45.0	58.0	57.7	1973-1982 bp	0.5	60.3
rps16_p3	CCTTTCAGGATCAGTCGTGG	CCGATCCTTGGCAGCATAAA	55.0	50.0	58.0	58.3	1961-1970 bp	0.5	60.0
rps16_p4	TCAGGATCAGTCGTGGTCTT	TGGTTTTCTTGCCGATCCTT	50.0	45.0	58.1	57.7	1968-1977 bp	0.5	59.8
rps16_p5	TCAGGATCAGTCGTGGTCTT	TTGGTTTTCTTGCCGATCCT	50.0	45.0	58.1	57.7	1969-1978 bp	0.5	59.8