Hotspot

ndhF

SSC · 135315-137528 · 2214 bp

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Pi 0.314

MarkerSeek score 75.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 1001
pi: 0.314
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGAACTGAGTGAGCCTTTGT	ACTTCCCATTTGGAGTTGCA	45.0	45.0	57.0	57.6	2941 bp	0.25	38.9
ndhF_p2	TGAACTGAGTGAGCCTTTGT	ATCCTGGGAAAACGTCAACG	45.0	50.0	57.0	58.5	2884 bp	0.25	38.1
ndhF_p3	TGAACTGAGTGAGCCTTTGT	GTTGCTGGCGTATTTGTCAA	45.0	45.0	57.0	57.3	2864 bp	0.25	36.4
ndhF_p4	ATGAACTGAGTGAGCCTTTGT	ACTTCCCATTTGGAGTTGCA	42.9	45.0	57.5	57.6	2942 bp	0.25	35.0
ndhF_p5	ATGAACTGAGTGAGCCTTTGT	ATCCTGGGAAAACGTCAACG	42.9	50.0	57.5	58.5	2885 bp	0.25	34.2