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trnL-UAG-rpl32

SSC · 133521-134099 · 579 bp

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Pi 0.402

MarkerSeek score 76.30

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 579
variable_sites: 315
pi: 0.402
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	CCTGAAGTCGCAAAAGCTTG	GTATCGTCACGCTCCAGTTT	50.0	50.0	58.0	58.0	1740 bp	0.25	49.6
trnL-UAG-rpl32_p2	CCTGAAGTCGCAAAAGCTTG	CGTCACGCTCCAGTTTATCA	50.0	50.0	58.0	58.0	1736 bp	0.25	49.6
trnL-UAG-rpl32_p3	AGTCGCAAAAGCTTGAGTCA	GTATCGTCACGCTCCAGTTT	45.0	50.0	58.0	58.0	1735 bp	0.25	49.6
trnL-UAG-rpl32_p4	AGTCGCAAAAGCTTGAGTCA	CGTCACGCTCCAGTTTATCA	45.0	50.0	58.0	58.0	1731 bp	0.25	49.6
trnL-UAG-rpl32_p5	CTGGGTGTGGTTATTGCACT	GTATCGTCACGCTCCAGTTT	50.0	50.0	58.1	58.0	1707 bp	0.25	49.2