Hotspot

ndhI-ndhG

SSC · 127958-128188 · 231 bp

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Pi 0.339

MarkerSeek score 72.10

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 110
pi: 0.339
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TGCTTATGATCGCCACGAAT	CCATCTCCCACAGTCCAAAG	45.0	55.0	57.8	57.9	666-808 bp	0.5	65.8
ndhI-ndhG_p2	ACAAACAGTACGAGCTGCAA	CCATCTCCCACAGTCCAAAG	45.0	55.0	58.1	57.9	1011-1153 bp	0.5	65.4
ndhI-ndhG_p3	AACAAACAGTACGAGCTGCA	CCATCTCCCACAGTCCAAAG	45.0	55.0	58.1	57.9	1012-1154 bp	0.5	65.3
ndhI-ndhG_p4	CAACAAACAGTACGAGCTGC	CCATCTCCCACAGTCCAAAG	50.0	55.0	57.7	57.9	1013-1155 bp	0.5	63.4
ndhI-ndhG_p5	ATAACCTCAGAGCGTTTCCG	CCATCTCCCACAGTCCAAAG	50.0	55.0	57.7	57.9	908 bp	0.25	46.7