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psbE-petL

LSC · 71632-72823 · 1192 bp

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Pi 0.0985

MarkerSeek score 84.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1192
variable_sites: 196
pi: 0.0985
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CGGTAGGTACAGCTAGTCCA	AAGCGCTCTTAGTTCAGCTC	55.0	50.0	58.0	58.0	2101-2127 bp	1.0	91.6
psbE-petL_p2	ACGGTAGGTACAGCTAGTCC	AAGCGCTCTTAGTTCAGCTC	55.0	50.0	58.0	58.0	2102-2128 bp	1.0	91.6
psbE-petL_p3	CGGTAGGTACAGCTAGTCCA	GCCGTTACAAACAATCCTGC	55.0	50.0	58.0	58.0	1867-1888 bp	1.0	91.4
psbE-petL_p4	ACGGTAGGTACAGCTAGTCC	GCCGTTACAAACAATCCTGC	55.0	50.0	58.0	58.0	1868-1889 bp	1.0	91.4
psbE-petL_p5	CGGTAGGTACAGCTAGTCCA	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.0	58.0	2078-2104 bp	1.0	91.3