Hotspot

trnE-UUC-trnT-GGU

LSC · 34200-35624 · 1425 bp

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Pi 0.13

MarkerSeek score 86.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1425
variable_sites: 299
pi: 0.13
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	ACTTTCCCAGTACCCTACCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1382-1645 bp	1.0	91.7
trnE-UUC-trnT-GGU_p2	ACTTTCCCAGTACCCTACCC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	1361-1624 bp	1.0	91.2
trnE-UUC-trnT-GGU_p3	ACTTTCCCAGTACCCTACCC	TGGCGTTACTCTACCACTGA	55.0	50.0	58.0	58.1	1358-1621 bp	1.0	91.0
trnE-UUC-trnT-GGU_p4	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1326-1589 bp	1.0	90.8
trnE-UUC-trnT-GGU_p5	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1324-1587 bp	1.0	90.8