Hotspot

psbM-trnD-GUC

LSC · 32578-33521 · 944 bp

Back to Wolffia

Pi 0.0903

MarkerSeek score 86.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 144
pi: 0.0903
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	35.0	50.0	52.0	58.5	958-1055 bp	1.0	52.0
psbM-trnD-GUC_p2	ATGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	33.3	50.0	52.7	58.5	959-1056 bp	1.0	52.0
psbM-trnD-GUC_p3	TGCGAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	33.3	50.0	52.7	58.5	958-1055 bp	1.0	52.0
psbM-trnD-GUC_p4	AATGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	31.8	50.0	53.6	58.5	960-1057 bp	1.0	52.0
psbM-trnD-GUC_p5	TGCGAGAATATTTACTTCCA	GTTCAATTGGTCAGAGCACC	35.0	50.0	52.0	57.0	959-1056 bp	1.0	52.0