Hotspot

rps16-trnQ-UUG

LSC · 6002-7476 · 1475 bp

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Pi 0.126

MarkerSeek score 84.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1475
variable_sites: 315
pi: 0.126
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GAACGAACCATGTCGAGACA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	57.9	57.9	2264-2291 bp	0.75	81.1
rps16-trnQ-UUG_p2	ACGAACCATGTCGAGACAAG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	57.9	57.9	2262-2289 bp	0.75	81.0
rps16-trnQ-UUG_p3	GAACGAACCATGTCGAGACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1625-1632 bp	0.75	80.9
rps16-trnQ-UUG_p4	ACGGATTCCAACACGTTTGA	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	2497-2538 bp	0.5	63.8
rps16-trnQ-UUG_p5	ACGGATTCCAACACGTTTGA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1864-1879 bp	0.5	63.8