primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	rps16	1	CCTTTCAGGATCAGTCGTGG	TGGTTTTCTTGCCGATCCTT	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.302100	4737	6001	1972-1981	0.500000	60.3
rps16_p2	rps16	2	CCTTTCAGGATCAGTCGTGG	TTGGTTTTCTTGCCGATCCT	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.302100	4737	6001	1973-1982	0.500000	60.3
rps16_p3	rps16	3	CCTTTCAGGATCAGTCGTGG	CCGATCCTTGGCAGCATAAA	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.336597	4737	6001	1961-1970	0.500000	60.0
rps16_p4	rps16	4	TCAGGATCAGTCGTGGTCTT	TGGTTTTCTTGCCGATCCTT	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.361633	4737	6001	1968-1977	0.500000	59.8
rps16_p5	rps16	5	TCAGGATCAGTCGTGGTCTT	TTGGTTTTCTTGCCGATCCT	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.361633	4737	6001	1969-1978	0.500000	59.8
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GAACGAACCATGTCGAGACA	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	57.9	57.9	12.2	0.0	0.116420	6002	7476	2264-2291	0.750000	81.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ACGAACCATGTCGAGACAAG	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	57.9	57.9	12.2	0.0	0.122087	6002	7476	2262-2289	0.750000	81.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GAACGAACCATGTCGAGACA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	12.2	16.9	0.136427	6002	7476	1625-1632	0.750000	80.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ACGGATTCCAACACGTTTGA	CTTACAGCAGCTTGCCAAAC	20	20	45.0	50.0	58.0	57.9	3.1	0.0	0.099797	6002	7476	2497-2538	0.500000	63.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ACGGATTCCAACACGTTTGA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.0	58.1	3.1	16.9	0.119804	6002	7476	1864-1879	0.500000	63.8
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACGAAAAGTTTGAGAGTAAGCA	20	22	50.0	36.4	57.9	56.2	1.4	0.0	3.809172	8170	8755	573-639	1.000000	61.5
psbK-psbI_p2	psbK-psbI	2	AGCATTTGTTTGGCAAGCTG	ACGAAAAGTTTGAGAGTAAGCA	20	22	45.0	36.4	58.1	56.2	15.2	0.0	3.879380	8170	8755	580-646	1.000000	61.0
psbK-psbI_p3	psbK-psbI	3	ATTTGTTTGGCAAGCTGCTG	ACGAAAAGTTTGAGAGTAAGCA	20	22	45.0	36.4	58.1	56.2	15.2	0.0	3.879380	8170	8755	577-643	1.000000	61.0
psbK-psbI_p4	psbK-psbI	4	CATTTGTTTGGCAAGCTGCT	ACGAAAAGTTTGAGAGTAAGCA	20	22	45.0	36.4	58.1	56.2	15.2	0.0	3.879380	8170	8755	578-644	1.000000	61.0
psbK-psbI_p5	psbK-psbI	5	TGGCAAGCTGCTGTAAGTTT	ACGAAAAGTTTGAGAGTAAGCA	20	22	45.0	36.4	58.3	56.2	1.4	0.0	4.061881	8170	8755	570-636	1.000000	59.5
trnC-GCA-petN_p1	trnC-GCA-petN	1	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.627590	30165	31169	776-1116	1.000000	87.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.5	58.2	14.7	0.0	0.676453	30165	31169	801-1141	1.000000	86.6
trnC-GCA-petN_p3	trnC-GCA-petN	3	GACATGGCCAAGTGGTAAGG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.5	58.2	20.2	0.0	0.687674	30165	31169	822-1162	1.000000	86.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	CGACATGGCCAAGTGGTAAG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.9	58.2	20.2	0.0	1.064652	30165	31169	823-1163	1.000000	83.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTAT	AGCCCAAGCGAGACTTACTA	21	20	47.6	50.0	58.0	58.2	14.7	0.0	1.183614	30165	31169	801-1141	1.000000	82.5
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	20	20	35.0	50.0	52.0	58.5	0.0	5.4	6.474201	32578	33521	958-1055	1.000000	52.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ATGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	21	20	33.3	50.0	52.7	58.5	0.0	5.4	6.746812	32578	33521	959-1056	1.000000	52.0
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TGCGAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	21	20	33.3	50.0	52.7	58.5	0.0	5.4	6.746812	32578	33521	958-1055	1.000000	52.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AATGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	22	20	31.8	50.0	53.6	58.5	0.0	5.4	6.858000	32578	33521	960-1057	1.000000	52.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TGCGAGAATATTTACTTCCA	GTTCAATTGGTCAGAGCACC	20	20	35.0	50.0	52.0	57.0	0.0	5.4	6.994354	32578	33521	959-1056	1.000000	52.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	ACTTTCCCAGTACCCTACCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.043412	34200	35624	1382-1645	1.000000	91.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	ACTTTCCCAGTACCCTACCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.101509	34200	35624	1361-1624	1.000000	91.2
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	ACTTTCCCAGTACCCTACCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.123664	34200	35624	1358-1621	1.000000	91.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	34200	35624	1326-1589	1.000000	90.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	34200	35624	1324-1587	1.000000	90.8
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	20	20	55.0	55.0	58.1	58.0	7.6	5.0	0.067776	35697	36895	1288-1341	1.000000	91.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	20	20	50.0	55.0	58.1	58.0	0.0	5.0	0.089930	35697	36895	1291-1344	1.000000	91.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	35697	36895	1297-1350	1.000000	90.1
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	CCACAAAAACGAAACGGTCC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	35697	36895	1298-1351	1.000000	90.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	35697	36895	1299-1352	1.000000	90.1
pafI-trnS-GGA_p1	pafI-trnS-GGA	1	ACACGACAGAAGGCATTGTT	GACTATTCCTGCAGCCAGAC	20	20	45.0	55.0	58.0	58.1	0.0	8.7	0.085936	48649	49481	1850-2369	1.000000	91.2
pafI-trnS-GGA_p2	pafI-trnS-GGA	2	AGTGTAGTGCTTCTTCCCCT	GACTATTCCTGCAGCCAGAC	20	20	50.0	55.0	58.0	58.1	0.0	8.7	0.084750	48649	49481	2002-2524	1.000000	91.0
pafI-trnS-GGA_p3	pafI-trnS-GGA	3	CTAGCCATCCTTCCTGCAAG	GACTATTCCTGCAGCCAGAC	20	20	55.0	55.0	58.0	58.1	0.0	8.7	0.088703	48649	49481	2271-2793	1.000000	90.8
pafI-trnS-GGA_p4	pafI-trnS-GGA	4	CCTGCCTGCCATTGGAATAT	GACTATTCCTGCAGCCAGAC	20	20	50.0	55.0	58.0	58.1	6.9	8.7	0.058625	48649	49481	1943-2460	0.750000	78.9
pafI-trnS-GGA_p5	pafI-trnS-GGA	5	CCTGCCTGCCATTGGAATAT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.0	58.1	6.9	0.0	0.099733	48649	49481	1258-1818	0.750000	77.8
rbcL-accD_p1	rbcL-accD	1	TGCCTGGTGTTATCCCTGTA	TAACTCCCGCTCTCATTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.179649	62693	63611	1286-1292	0.500000	60.7
rbcL-accD_p2	rbcL-accD	2	TGCACTGACCGAGATCTTTG	TAACTCCCGCTCTCATTCCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.223968	62693	63611	1234-1240	0.500000	60.4
rbcL-accD_p3	rbcL-accD	3	GCACTACGTATGTCTGGTGG	TAACTCCCGCTCTCATTCCA	20	20	55.0	50.0	58.1	58.1	11.1	0.0	0.216789	62693	63611	1449-1455	0.500000	60.2
rbcL-accD_p4	rbcL-accD	4	GGTGAGCGTGAGATGACTTT	TAACTCCCGCTCTCATTCCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.225413	62693	63611	1389-1395	0.500000	60.2
rbcL-accD_p5	rbcL-accD	5	TTATCCGTGAAGCTGCGAAA	TAACTCCCGCTCTCATTCCA	20	20	45.0	50.0	57.9	58.1	7.1	0.0	0.226860	62693	63611	1067-1067	0.250000	48.2
psbE-petL_p1	psbE-petL	1	CGGTAGGTACAGCTAGTCCA	AAGCGCTCTTAGTTCAGCTC	20	20	55.0	50.0	58.0	58.0	0.0	12.1	0.051641	71632	72823	2101-2127	1.000000	91.6
psbE-petL_p2	psbE-petL	2	ACGGTAGGTACAGCTAGTCC	AAGCGCTCTTAGTTCAGCTC	20	20	55.0	50.0	58.0	58.0	0.0	12.1	0.053085	71632	72823	2102-2128	1.000000	91.6
psbE-petL_p3	psbE-petL	3	CGGTAGGTACAGCTAGTCCA	GCCGTTACAAACAATCCTGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.071256	71632	72823	1867-1888	1.000000	91.4
psbE-petL_p4	psbE-petL	4	ACGGTAGGTACAGCTAGTCC	GCCGTTACAAACAATCCTGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.072700	71632	72823	1868-1889	1.000000	91.4
psbE-petL_p5	psbE-petL	5	CGGTAGGTACAGCTAGTCCA	AGAACGTGGGTCTCCAAAAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.085272	71632	72823	2078-2104	1.000000	91.3
ndhH_p1	ndhH	1	ACTTCCCATTTGGAGTTGCA	TCAGGTCCAATACGCTGTTG	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.473370	124140	125327	1705-1708	0.500000	62.1
ndhH_p2	ndhH	2	ACTTCCCATTTGGAGTTGCA	CAGGTCCAATACGCTGTTGT	20	20	45.0	50.0	57.6	58.2	0.0	0.0	0.571653	124140	125327	1704-1707	0.500000	61.3
ndhH_p3	ndhH	3	ACTTCCCATTTGGAGTTGCA	CCCCCTAGATGGAAGGACAT	20	20	45.0	55.0	57.6	57.9	0.0	0.0	0.504624	124140	125327	1793-1793	0.250000	46.0
ndhH_p4	ndhH	4	ACTTCCCATTTGGAGTTGCA	ATACGACTGCAATAGCTGGC	20	20	45.0	50.0	57.6	58.1	0.0	0.0	0.508984	124140	125327	1836-1836	0.250000	45.9
ndhH_p5	ndhH	5	ACTTCCCATTTGGAGTTGCA	CTGTTGTATCGCGGCAGATA	20	20	45.0	50.0	57.6	57.9	0.0	6.1	0.514265	124140	125327	1694-1694	0.250000	45.9
ndhI-ndhG_p1	ndhI-ndhG	1	TGCTTATGATCGCCACGAAT	CCATCTCCCACAGTCCAAAG	20	20	45.0	55.0	57.8	57.9	0.0	0.0	0.358743	127958	128188	666-808	0.500000	65.8
ndhI-ndhG_p2	ndhI-ndhG	2	ACAAACAGTACGAGCTGCAA	CCATCTCCCACAGTCCAAAG	20	20	45.0	55.0	58.1	57.9	1.0	0.0	0.171697	127958	128188	1011-1153	0.500000	65.4
ndhI-ndhG_p3	ndhI-ndhG	3	AACAAACAGTACGAGCTGCA	CCATCTCCCACAGTCCAAAG	20	20	45.0	55.0	58.1	57.9	1.0	0.0	0.171697	127958	128188	1012-1154	0.500000	65.3
ndhI-ndhG_p4	ndhI-ndhG	4	CAACAAACAGTACGAGCTGC	CCATCTCCCACAGTCCAAAG	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.420425	127958	128188	1013-1155	0.500000	63.4
ndhI-ndhG_p5	ndhI-ndhG	5	ATAACCTCAGAGCGTTTCCG	CCATCTCCCACAGTCCAAAG	20	20	50.0	55.0	57.7	57.9	8.9	0.0	0.415054	127958	128188	908-908	0.250000	46.7
ndhE-psaC_p1	ndhE-psaC	1	GTCGAAGCATGGTTAGAGCA	GAAAAATGAAACAGCCCGCA	20	20	50.0	45.0	58.0	57.9	18.9	0.0	0.157581	129452	130274	1507-1510	0.500000	69.8
ndhE-psaC_p2	ndhE-psaC	2	TCGAAGCATGGTTAGAGCAC	GAAAAATGAAACAGCCCGCA	20	20	50.0	45.0	58.0	57.9	15.3	0.0	0.157581	129452	130274	1506-1509	0.500000	69.8
ndhE-psaC_p3	ndhE-psaC	3	GTCGAAGCATGGTTAGAGCA	TATTTCCTCTGTGCGGGAGA	20	20	50.0	50.0	58.0	58.1	18.9	2.6	0.149504	129452	130274	1528-1528	0.250000	48.8
ndhE-psaC_p4	ndhE-psaC	4	TCGAAGCATGGTTAGAGCAC	TATTTCCTCTGTGCGGGAGA	20	20	50.0	50.0	58.0	58.1	15.3	2.6	0.149504	129452	130274	1527-1527	0.250000	48.8
ndhE-psaC_p5	ndhE-psaC	5	CGAAGCATGGTTAGAGCACT	TATTTCCTCTGTGCGGGAGA	20	20	50.0	50.0	58.0	58.1	10.7	2.6	0.155095	129452	130274	1526-1526	0.250000	48.8
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	CCTGAAGTCGCAAAAGCTTG	GTATCGTCACGCTCCAGTTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049934	133521	134099	1740-1740	0.250000	49.6
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CCTGAAGTCGCAAAAGCTTG	CGTCACGCTCCAGTTTATCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051305	133521	134099	1736-1736	0.250000	49.6
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGTCGCAAAAGCTTGAGTCA	GTATCGTCACGCTCCAGTTT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054271	133521	134099	1735-1735	0.250000	49.6
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AGTCGCAAAAGCTTGAGTCA	CGTCACGCTCCAGTTTATCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.055642	133521	134099	1731-1731	0.250000	49.6
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CTGGGTGTGGTTATTGCACT	GTATCGTCACGCTCCAGTTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.098131	133521	134099	1707-1707	0.250000	49.2
rpl32-ndhF_p1	rpl32-ndhF	1	ACTGTCCTGTGGAGATCGAT	CCCACATACGACGACTGTTT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.226049	134274	135314	1281-1289	0.500000	70.2
rpl32-ndhF_p2	rpl32-ndhF	2	ACTGTCCTGTGGAGATCGAT	AAGGGGAAATTGTGCGGATT	20	20	50.0	45.0	57.8	58.1	0.0	0.0	0.214311	134274	135314	1892-1900	0.500000	69.2
rpl32-ndhF_p3	rpl32-ndhF	3	ACTGTCCTGTGGAGATCGAT	AGGGGAAATTGTGCGGATTT	20	20	50.0	45.0	57.8	58.1	0.0	0.0	0.214311	134274	135314	1891-1899	0.500000	69.2
rpl32-ndhF_p4	rpl32-ndhF	4	ACTGTCCTGTGGAGATCGAT	TTGGTCGGGTGAACCAAAAA	20	20	50.0	45.0	57.8	58.2	0.0	5.3	0.302851	134274	135314	1668-1676	0.500000	68.8
rpl32-ndhF_p5	rpl32-ndhF	5	ACTGTCCTGTGGAGATCGAT	AGGAAATGCCCACATACGAC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.189889	134274	135314	1297-1297	0.250000	48.5
ndhF_p1	ndhF	1	TGAACTGAGTGAGCCTTTGT	ACTTCCCATTTGGAGTTGCA	20	20	45.0	45.0	57.0	57.6	0.0	0.0	1.388752	135315	137528	2941-2941	0.250000	38.9
ndhF_p2	ndhF	2	TGAACTGAGTGAGCCTTTGT	ATCCTGGGAAAACGTCAACG	20	20	45.0	50.0	57.0	58.5	0.0	0.1	1.489280	135315	137528	2884-2884	0.250000	38.1
ndhF_p3	ndhF	3	TGAACTGAGTGAGCCTTTGT	GTTGCTGGCGTATTTGTCAA	20	20	45.0	45.0	57.0	57.3	0.0	0.0	1.706119	135315	137528	2864-2864	0.250000	36.4
ndhF_p4	ndhF	4	ATGAACTGAGTGAGCCTTTGT	ACTTCCCATTTGGAGTTGCA	21	20	42.9	45.0	57.5	57.6	0.0	0.0	1.878838	135315	137528	2942-2942	0.250000	35.0
ndhF_p5	ndhF	5	ATGAACTGAGTGAGCCTTTGT	ATCCTGGGAAAACGTCAACG	21	20	42.9	50.0	57.5	58.5	0.0	0.1	1.979366	135315	137528	2885-2885	0.250000	34.2