Hotspot

ycf1

SSC · 124658-130327 · 5670 bp

Back to Withania

Pi 0.0199

MarkerSeek score 60.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5670
variable_sites: 335
pi: 0.0199
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCCGCATAGAGCCTTTAAT	CGCTCCCTAATGAACTGGAC	50.0	55.0	58.1	58.1	826 bp	0.875	76.5
ycf1_p2	GCCCGCATAGAGCCTTTAAT	AAGCGCTCCCTAATGAACTG	50.0	50.0	58.1	58.0	829 bp	0.5	59.1
ycf1_p3	TTCGCTCTTCCCTGTTCTTG	AAGCGCTCCCTAATGAACTG	50.0	50.0	57.8	58.0	493 bp	0.5	58.6
ycf1_p4	TAAAGCCACTTCCTCGTTGG	AAGCGCTCCCTAATGAACTG	50.0	50.0	57.8	58.0	770 bp	0.5	58.5
ycf1_p5	GCTCTTCCCTGTTCTTGGTC	AAGCGCTCCCTAATGAACTG	55.0	50.0	58.2	58.0	490 bp	0.5	58.3