Hotspot

clpP

LSC · 71574-73575 · 2002 bp

Back to Withania

Pi 0.0189

MarkerSeek score 59.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2002
variable_sites: 114
pi: 0.0189
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TTTCTTGCTTTTGTGCCAGC	ACGAACATACCCTGTCTCCA	45.0	50.0	58.1	58.1	2897-2965 bp	1.0	84.6
clpP_p2	CCCTCTTAGTCCGTTCTTGC	TTTCTATGAGCGAATGGGGC	55.0	50.0	58.0	58.0	2793-2837 bp	0.75	71.7
clpP_p3	TAGCTGACCCTCTTAGTCCG	TTTCTATGAGCGAATGGGGC	55.0	50.0	57.9	58.0	2800-2844 bp	0.75	71.4
clpP_p4	TTTCTTGCTTTTGTGCCAGC	TTTCTATGAGCGAATGGGGC	45.0	50.0	58.1	58.0	2509-2551 bp	0.75	71.2
clpP_p5	TCTTGCTAGATTGGGAGCCT	TTTCTATGAGCGAATGGGGC	50.0	50.0	58.1	58.0	2779-2823 bp	0.75	70.9