Hotspot

ndhF-rpl32

SSC · 113075-113883 · 809 bp

Back to Withania

Pi 0.0294
MarkerSeek score 74.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
809
variable_sites
72
pi
0.0294
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGTCGATCAAGTATCCGA AATCAAACTCCCCACCTTGG 50.0 50.0 57.9 57.7 1502-1571 bp 1.0 86.3
ndhF-rpl32_p2 TGGGTCGATCAAGTATCCGA CAAACTCCCCACCTTGGAAT 50.0 50.0 57.9 57.7 1499-1568 bp 1.0 86.3
ndhF-rpl32_p3 TGGGTCGATCAAGTATCCGA ATGCTGCCCAATATCCCTTC 50.0 50.0 57.9 57.7 1147-1167 bp 0.875 76.6
ndhF-rpl32_p4 GTGGGTCGATCAAGTATCCG ATGCTGCCCAATATCCCTTC 55.0 50.0 57.9 57.7 1148-1168 bp 0.875 76.0
ndhF-rpl32_p5 ATTGGGCGGATTTAGCAACT ATGCTGCCCAATATCCCTTC 45.0 50.0 57.9 57.7 1604-1624 bp 0.875 74.9