Hotspot

petD-rpoA

LSC · 79353-79531 · 179 bp

Back to Withania

Pi 0.0383

MarkerSeek score 62.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 22
pi: 0.0383
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CCTTTGGTTGGGCATTGGT	TGAACATTTTCGTAGCGAAGA	52.6	38.1	58.5	55.9	330-335 bp	1.0	49.0
petD-rpoA_p2	CTTTGGTTGGGCATTGGTG	TGAACATTTTCGTAGCGAAGA	52.6	38.1	57.4	55.9	329-334 bp	1.0	48.5
petD-rpoA_p3	CCTTTGGTTGGGCATTGGT	TTGAACATTTTCGTAGCGAAGA	52.6	36.4	58.5	56.7	331-336 bp	1.0	46.8
petD-rpoA_p4	CTTTGGTTGGGCATTGGTG	TTGAACATTTTCGTAGCGAAGA	52.6	36.4	57.4	56.7	330-335 bp	1.0	46.3
petD-rpoA_p5	CCTTTGGTTGGGCATTGGTG	TGAACATTTTCGTAGCGAAGA	55.0	38.1	60.0	55.9	330-335 bp	1.0	45.8