Hotspot

rpl33-rps18

LSC · 69409-69600 · 192 bp

Back to Withania

Pi 0.0434

MarkerSeek score 62.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 25
pi: 0.0434
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CAAGGGGAAAGATGTCCGAG	CAGTAATTCGCGAAAGGGGA	55.0	50.0	58.0	58.0	1031-1160 bp	1.0	88.7
rpl33-rps18_p2	AAAGGAGGTTCATGGCCAAG	CAGTAATTCGCGAAAGGGGA	50.0	50.0	58.1	58.0	1047-1176 bp	1.0	88.3
rpl33-rps18_p3	ATCCCTCTCCCCCTCTTTTC	CAGTAATTCGCGAAAGGGGA	55.0	50.0	58.1	58.0	1417-1553 bp	1.0	87.7
rpl33-rps18_p4	GAATCCCTCTCCCCCTCTTT	CAGTAATTCGCGAAAGGGGA	55.0	50.0	58.1	58.0	1419-1555 bp	1.0	87.7
rpl33-rps18_p5	ATGCGTTGACATTCCCCTTT	CAGTAATTCGCGAAAGGGGA	45.0	50.0	58.1	58.0	1506-1601 bp	0.625	72.5