Hotspot

psaJ-rpl33

LSC · 68758-69207 · 450 bp

Back to Withania

Pi 0.0186

MarkerSeek score 60.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 22
pi: 0.0186
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGCACCAGTACTAAGTACGC	CTCGGACATCTTTCCCCTTG	55.0	55.0	58.1	58.0	569-602 bp	1.0	84.9
psaJ-rpl33_p2	ACCCGTGACATTTTGTACCC	CTCGGACATCTTTCCCCTTG	50.0	55.0	58.1	58.0	1082-1124 bp	1.0	83.6
psaJ-rpl33_p3	CCCGTGACATTTTGTACCCA	CTCGGACATCTTTCCCCTTG	50.0	55.0	58.1	58.0	1081-1123 bp	1.0	83.6
psaJ-rpl33_p4	ACAGGATTTGAACCCGTGAC	CTCGGACATCTTTCCCCTTG	50.0	55.0	58.1	58.0	1093-1135 bp	1.0	83.5
psaJ-rpl33_p5	ATGCGTTGACATTCCCCTTT	CTCGGACATCTTTCCCCTTG	45.0	55.0	58.1	58.0	495-528 bp	0.625	70.1