Hotspot

petA-psbJ

LSC · 64734-65438 · 705 bp

Back to Withania

Pi 0.0439

MarkerSeek score 77.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 82
pi: 0.0439
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGATTTGGTCAGGGAGATG	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1222-1603 bp	1.0	86.1
petA-psbJ_p2	CGGATTTGGTCAGGGAGATG	ACGACACAATCAAACCCGAA	55.0	45.0	58.0	58.0	1225-1606 bp	1.0	86.1
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	1533-1914 bp	1.0	85.2
petA-psbJ_p4	CGGATTTGGTCAGGGAGATG	GAGCCGACAAGGAATTCCAT	55.0	50.0	58.0	58.0	1455-1836 bp	1.0	85.2
petA-psbJ_p5	GCTCCTCCCGATCGTATTTC	TGCGGGTTGGTTATTTGTCA	55.0	45.0	57.9	58.0	1985-2366 bp	1.0	84.1