Hotspot

trnL-UAA-trnF-GAA

LSC · 49283-49582 · 300 bp

Back to Withania

Pi 0.0549

MarkerSeek score 73.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 300
variable_sites: 39
pi: 0.0549
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	380-580 bp	1.0	86.4
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	395-595 bp	1.0	85.9
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	398-598 bp	1.0	85.4
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	AATCATGTGCCAGGAACCAG	50.0	50.0	57.5	58.2	401-601 bp	1.0	85.3
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	396-596 bp	1.0	83.6