Hotspot

rpoB-trnC-GCA

LSC · 27386-28698 · 1313 bp

Back to Withania

Pi 0.0178

MarkerSeek score 62.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1313
variable_sites: 69
pi: 0.0178
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	TTGCGAAGTCTTGATAGCCC	50.0	50.0	58.0	58.0	2103-2136 bp	1.0	84.7
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	GGCGCAGAGTCAAAAAGAAC	50.0	50.0	58.0	58.0	2478-2511 bp	1.0	84.3
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	TTCTGCTGGGGAATCATTCG	50.0	50.0	58.0	58.0	1950-1991 bp	1.0	84.3
rpoB-trnC-GCA_p4	AGAAAATACCGGATCGCCAC	TTGCGAAGTCTTGATAGCCC	50.0	50.0	58.1	58.0	2311-2344 bp	1.0	84.2
rpoB-trnC-GCA_p5	CAGAAAATACCGGATCGCCA	TTGCGAAGTCTTGATAGCCC	50.0	50.0	58.1	58.0	2312-2345 bp	1.0	84.2