Hotspot

rps16-trnQ

LSC · 6160-7383 · 1224 bp

Back to Withania

Pi 0.0246

MarkerSeek score 68.50

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1224
variable_sites: 85
pi: 0.0246
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	TGGCAGCAACATACCCTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1512-1715 bp	1.0	88.2
rps16-trnQ_p2	TGGCAGCAACATACCCTTTT	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	2076-2287 bp	1.0	87.9
rps16-trnQ_p3	CCCTCGTGAAACACTTTGGA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2018-2229 bp	1.0	87.7
rps16-trnQ_p4	CCAATGATGTCGAGCCAAGA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1880-1975 bp	0.875	79.7
rps16-trnQ_p5	AGAACCAATGATGTCGAGCC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1884-1979 bp	0.875	79.7