Hotspot

ycf4

Genome · 62582-63220 · 639 bp

Back to Wisteriopsis

Pi 0.00156

MarkerSeek score 46.70

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: Genome
length_bp: 639
variable_sites: 1
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ATTTCCGGCAATTGCAATGG	TGGTTGTGCGTCTTGATCAA	45.0	45.0	58.0	58.0	1499 bp	1.0	80.1
ycf4_p2	ATTTCCGGCAATTGCAATGG	TTGGTTGTGCGTCTTGATCA	45.0	45.0	58.0	58.0	1500 bp	1.0	80.1
ycf4_p3	GAGGGCGAAATTCATCCAGT	TGGTTGTGCGTCTTGATCAA	50.0	45.0	58.0	58.0	1920 bp	1.0	79.8
ycf4_p4	GAGGGCGAAATTCATCCAGT	TTGGTTGTGCGTCTTGATCA	50.0	45.0	58.0	58.0	1921 bp	1.0	79.8
ycf4_p5	GGCAATTGCAATGGCTTCTT	TGGTTGTGCGTCTTGATCAA	45.0	45.0	57.9	58.0	1493 bp	1.0	79.4