Hotspot

petA

Genome · 64456-65418 · 963 bp

Back to Wisteriopsis

Pi 0.00104

MarkerSeek score 47.40

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: Genome
length_bp: 963
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGGGAACTAATGCCAGTAACC	ATGTTGATAAATCCGCGTAC	47.6	40.0	57.4	53.9	1048 bp	1.0	40.1
petA_p2	GGGAACTAATGCCAGTAACCT	ATGTTGATAAATCCGCGTAC	47.6	40.0	57.4	53.9	1047 bp	1.0	40.1
petA_p3	GGGAACTAATGCCAGTAACC	ATGTTGATAAATCCGCGTAC	50.0	40.0	55.8	53.9	1047 bp	1.0	40.1
petA_p4	AGCAGAATCATGGATAGGGAAC	ATGTTGATAAATCCGCGTAC	45.5	40.0	57.6	53.9	1063 bp	1.0	40.1
petA_p5	GGGAACTAATGCCAGTAACCT	TATGTTGATAAATCCGCGTAC	47.6	38.1	57.4	53.9	1048 bp	1.0	40.1