Hotspot

matK

Genome · 2211-3731 · 1521 bp

Back to Wisteriopsis

Pi 0.00131
MarkerSeek score 59.50
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
Genome
length_bp
1521
variable_sites
2
pi
0.00131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 ACAAAGTCTCATAACATAACCA CCTTGTTTTGACTGTATCGCA 31.8 42.9 53.1 56.8 1625 bp 1.0 40.1
matK_p2 ACAAAGTCTCATAACATAACCA ACCTTGTTTTGACTGTATCGC 31.8 42.9 53.1 56.8 1626 bp 1.0 40.1
matK_p3 ACAAAGTCTCATAACATAACCA CCTTGTTTTGACTGTATCGCAC 31.8 45.5 53.1 58.2 1625 bp 1.0 40.1
matK_p4 ACAAAGTCTCATAACATAACCAA CCTTGTTTTGACTGTATCGCA 30.4 42.9 53.9 56.8 1625 bp 1.0 40.1
matK_p5 ACAAAGTCTCATAACATAACCAA ACCTTGTTTTGACTGTATCGC 30.4 42.9 53.9 56.8 1626 bp 1.0 40.1