Hotspot

ycf4-cemA

Genome · 63221-63554 · 334 bp

Back to Wisteriopsis

Pi 0.0269

MarkerSeek score 68.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: Genome
length_bp: 334
variable_sites: 9
pi: 0.0269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TCTAGGACTCCAGCCAAAGT	TAAAAGAGATGCACCAGGGC	50.0	50.0	57.7	57.9	488 bp	1.0	77.8
ycf4-cemA_p2	AAAAGCTTGGGACTTGTCCA	TAAAAGAGATGCACCAGGGC	45.0	50.0	57.5	57.9	462 bp	1.0	76.9
ycf4-cemA_p3	TCTTGCGTGTACCAATGGAA	TAAAAGAGATGCACCAGGGC	45.0	50.0	57.4	57.9	439 bp	1.0	76.2
ycf4-cemA_p4	TTCTTGCGTGTACCAATGGA	TAAAAGAGATGCACCAGGGC	45.0	50.0	57.4	57.9	440 bp	1.0	76.2
ycf4-cemA_p5	TCTAGGACTCCAGCCAAAGT	GCACCAGGGCAAAAAGACTA	50.0	50.0	57.7	58.4	478 bp	1.0	75.8