Hotspot

psbK-trnQ-UUG

Genome · 57086-57804 · 719 bp

Back to Wisteriopsis

Pi 0.00139

MarkerSeek score 59.50

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 719
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	CGTAGGCTTCAGGCAATTTC	CGAATAACGGGACCAAAACC	50.0	50.0	57.2	57.1	858 bp	1.0	66.2
psbK-trnQ-UUG_p2	GTAGGCTTCAGGCAATTTCG	CGAATAACGGGACCAAAACC	50.0	50.0	57.2	57.1	857 bp	1.0	66.2
psbK-trnQ-UUG_p3	TAGGCTTCAGGCAATTTCGC	CGAATAACGGGACCAAAACC	50.0	50.0	58.9	57.1	856 bp	1.0	65.6
psbK-trnQ-UUG_p4	GCGTAGGCTTCAGGCAATTT	CGAATAACGGGACCAAAACC	50.0	50.0	59.2	57.1	859 bp	1.0	63.3
psbK-trnQ-UUG_p5	GTAGGCTTCAGGCAATTTCG	GAATAACGGGACCAAAACCC	50.0	50.0	57.2	56.7	856 bp	1.0	63.0