Hotspot

atpI-atpH

Genome · 49056-50473 · 1418 bp

Back to Wisteriopsis

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 1418
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.3	58.0	1580 bp	1.0	73.9
atpI-atpH_p2	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	45.0	50.0	57.2	58.0	1616 bp	1.0	73.7
atpI-atpH_p3	GGTGAATCCATGGAAGGTCA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.2	58.0	1565 bp	1.0	73.4
atpI-atpH_p4	GCTGCGGCTTATATAGGTGA	AACAGAAGCGGCAGAAATCA	50.0	45.0	57.3	58.1	1490 bp	1.0	73.3
atpI-atpH_p5	ACAAGCGGTATTCAAGCTCT	AACAGAAGCGGCAGAAATCA	45.0	45.0	57.2	58.1	1526 bp	1.0	73.1