Hotspot

matK-trnK-UUU

Genome · 3732-4460 · 729 bp

Back to Wisteriopsis

Pi 0.00137

MarkerSeek score 59.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: Genome
length_bp: 729
variable_sites: 1
pi: 0.00137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGTCCTGTTGGCGAGATCTA	TCAATGGTAGAGTACTCGGCT	50.0	47.6	57.9	58.0	807 bp	1.0	70.8
matK-trnK-UUU_p2	GAAGTCCTGTTGGCGAGATC	TCAATGGTAGAGTACTCGGCT	55.0	47.6	58.4	58.0	809 bp	1.0	69.2
matK-trnK-UUU_p3	AGTCCTGTTGGCGAGATCTA	AGGGTTGCTAACTCAATGGT	50.0	45.0	57.9	56.7	819 bp	1.0	68.7
matK-trnK-UUU_p4	AGTCCTGTTGGCGAGATCTA	CTCAATGGTAGAGTACTCGGC	50.0	52.4	57.9	57.6	808 bp	1.0	68.1
matK-trnK-UUU_p5	GAAGTCCTGTTGGCGAGATC	AGGGTTGCTAACTCAATGGT	55.0	45.0	58.4	56.7	821 bp	1.0	67.1