primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	AGAAATGACTTCGCTATGAT	TGCGATTGGATATACACATCCT	20	22	35.0	40.9	52.1	57.1	0.0	3.9	8.811156	1865	4497	2731-2731	1.000000	40.1
trnK-UUU_p2	trnK-UUU	2	ACCTCATTTCATCATTCATTCA	TGCGATTGGATATACACATCCT	22	22	31.8	40.9	53.8	57.1	0.0	3.9	9.129941	1865	4497	2780-2781	1.000000	40.1
trnK-UUU_p3	trnK-UUU	3	AGAAATGACTTCGCTATGAT	TTGCGATTGGATATACACATCCT	20	23	35.0	39.1	52.1	57.8	0.0	3.9	9.148567	1865	4497	2732-2732	1.000000	40.1
trnK-UUU_p4	trnK-UUU	4	AGAAATGACTTCGCTATGAT	TGCGATTGGATATACACATCC	20	21	35.0	42.9	52.1	55.6	0.0	0.0	9.293209	1865	4497	2731-2731	1.000000	40.1
trnK-UUU_p5	trnK-UUU	5	ACCTCATTTCATCATTCATTCAA	TGCGATTGGATATACACATCCT	23	22	30.4	40.9	54.6	57.1	0.0	3.9	9.317359	1865	4497	2780-2781	1.000000	40.1
matK_p1	matK	1	ACAAAGTCTCATAACATAACCA	CCTTGTTTTGACTGTATCGCA	22	21	31.8	42.9	53.1	56.8	0.0	0.0	9.063845	2211	3731	1625-1625	1.000000	40.1
matK_p2	matK	2	ACAAAGTCTCATAACATAACCA	ACCTTGTTTTGACTGTATCGC	22	21	31.8	42.9	53.1	56.8	0.0	0.0	9.065879	2211	3731	1626-1626	1.000000	40.1
matK_p3	matK	3	ACAAAGTCTCATAACATAACCA	CCTTGTTTTGACTGTATCGCAC	22	22	31.8	45.5	53.1	58.2	0.0	0.0	9.085984	2211	3731	1625-1625	1.000000	40.1
matK_p4	matK	4	ACAAAGTCTCATAACATAACCAA	CCTTGTTTTGACTGTATCGCA	23	21	30.4	42.9	53.9	56.8	0.0	0.0	9.223682	2211	3731	1625-1625	1.000000	40.1
matK_p5	matK	5	ACAAAGTCTCATAACATAACCAA	ACCTTGTTTTGACTGTATCGC	23	21	30.4	42.9	53.9	56.8	0.0	0.0	9.225716	2211	3731	1626-1626	1.000000	40.1
matK-trnK-UUU_p1	matK-trnK-UUU	1	AGTCCTGTTGGCGAGATCTA	TCAATGGTAGAGTACTCGGCT	20	21	50.0	47.6	57.9	58.0	0.0	17.1	1.159701	3732	4460	807-807	1.000000	70.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	GAAGTCCTGTTGGCGAGATC	TCAATGGTAGAGTACTCGGCT	20	21	55.0	47.6	58.4	58.0	0.0	17.1	1.368114	3732	4460	809-809	1.000000	69.2
matK-trnK-UUU_p3	matK-trnK-UUU	3	AGTCCTGTTGGCGAGATCTA	AGGGTTGCTAACTCAATGGT	20	20	50.0	45.0	57.9	56.7	0.0	2.5	1.421044	3732	4460	819-819	1.000000	68.7
matK-trnK-UUU_p4	matK-trnK-UUU	4	AGTCCTGTTGGCGAGATCTA	CTCAATGGTAGAGTACTCGGC	20	21	50.0	52.4	57.9	57.6	0.0	17.1	1.502562	3732	4460	808-808	1.000000	68.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	GAAGTCCTGTTGGCGAGATC	AGGGTTGCTAACTCAATGGT	20	20	55.0	45.0	58.4	56.7	0.0	2.5	1.629458	3732	4460	821-821	1.000000	67.1
rbcL-atpB_p1	rbcL-atpB	1	AACACCAGCTTTGAACCCAA	GGCGATACGTCCCAGATTTT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.191233	7191	7972	904-905	1.000000	78.6
rbcL-atpB_p2	rbcL-atpB	2	AACACCAGCTTTGAACCCAA	CCTGGGGGAAAAGCTACATC	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.199291	7191	7972	945-946	1.000000	78.6
rbcL-atpB_p3	rbcL-atpB	3	AACACCAGCTTTGAACCCAA	GGACCGATTATTTGGGCGAT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.248489	7191	7972	918-919	1.000000	78.2
rbcL-atpB_p4	rbcL-atpB	4	GCTTTGAACCCAACACTTGC	GGCGATACGTCCCAGATTTT	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.479722	7191	7972	897-898	1.000000	76.3
rbcL-atpB_p5	rbcL-atpB	5	GCTTTGAACCCAACACTTGC	CCTGGGGGAAAAGCTACATC	20	20	50.0	55.0	58.4	57.9	0.0	0.0	0.487780	7191	7972	938-939	1.000000	76.3
trnG-GCC-psbZ_p1	trnG-GCC-psbZ	1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGATGGTTGGTCG	20	20	50.0	55.0	57.8	58.0	1.1	0.0	0.185568	26129	26942	968-968	1.000000	78.7
trnG-GCC-psbZ_p2	trnG-GCC-psbZ	2	TACATAAAAAGGCAGGGGCG	CTTCTCCTGATGGTTGGTCG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.258054	26129	26942	1008-1008	1.000000	78.1
trnG-GCC-psbZ_p3	trnG-GCC-psbZ	3	CGTCTTCTCCTTGGCAAAGA	TTGCTTCTCCTGATGGTTGG	20	20	50.0	50.0	57.8	57.8	1.1	0.0	0.374855	26129	26942	971-971	1.000000	77.2
trnG-GCC-psbZ_p4	trnG-GCC-psbZ	4	CGTCTTCTCCTTGGCAAAGA	TCCTGATGGTTGGTCGAGTA	20	20	50.0	50.0	57.8	57.8	1.1	0.0	0.395214	26129	26942	964-964	1.000000	77.0
trnG-GCC-psbZ_p5	trnG-GCC-psbZ	5	CGTCTTCTCCTTGGCAAAGA	GCTTCTCCTGATGGTTGGTC	20	20	50.0	55.0	57.8	58.3	1.1	0.0	0.425837	26129	26942	969-969	1.000000	76.8
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.165150	31772	32591	961-962	1.000000	78.8
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	GGCGTTACTCTACCACACTG	TTTCGTAGTACCCTACCCCC	20	20	55.0	55.0	58.0	57.8	0.0	0.0	0.167010	31772	32591	936-937	1.000000	78.7
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	GAACCGATGACTTACGCCTT	CGTTGCCTCCTTGAAAGAGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.175803	31772	32591	924-925	1.000000	78.7
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	GGCGTTACTCTACCACACTG	CGTTGCCTCCTTGAAAGAGA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.177663	31772	32591	899-900	1.000000	78.7
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	CCATGGCGTTACTCTACCAC	TTTCGTAGTACCCTACCCCC	20	20	55.0	55.0	58.1	57.8	0.0	0.0	0.223247	31772	32591	940-941	1.000000	78.3
atpI-atpH_p1	atpI-atpH	1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	20	20	50.0	50.0	57.3	58.0	11.2	0.0	0.773474	49056	50473	1580-1580	1.000000	73.9
atpI-atpH_p2	atpI-atpH	2	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.800002	49056	50473	1616-1616	1.000000	73.7
atpI-atpH_p3	atpI-atpH	3	GGTGAATCCATGGAAGGTCA	CGCAATCCCTTCTACAGCTT	20	20	50.0	50.0	57.2	58.0	20.7	0.0	0.836783	49056	50473	1565-1565	1.000000	73.4
atpI-atpH_p4	atpI-atpH	4	GCTGCGGCTTATATAGGTGA	AACAGAAGCGGCAGAAATCA	20	20	50.0	45.0	57.3	58.1	11.2	1.4	0.847416	49056	50473	1490-1490	1.000000	73.3
atpI-atpH_p5	atpI-atpH	5	ACAAGCGGTATTCAAGCTCT	AACAGAAGCGGCAGAAATCA	20	20	45.0	45.0	57.2	58.1	0.0	1.4	0.873944	49056	50473	1526-1526	1.000000	73.1
psbK-trnQ-UUG_p1	psbK-trnQ-UUG	1	CGTAGGCTTCAGGCAATTTC	CGAATAACGGGACCAAAACC	20	20	50.0	50.0	57.2	57.1	2.9	0.0	1.738631	57086	57804	858-858	1.000000	66.2
psbK-trnQ-UUG_p2	psbK-trnQ-UUG	2	GTAGGCTTCAGGCAATTTCG	CGAATAACGGGACCAAAACC	20	20	50.0	50.0	57.2	57.1	2.9	0.0	1.738631	57086	57804	857-857	1.000000	66.2
psbK-trnQ-UUG_p3	psbK-trnQ-UUG	3	TAGGCTTCAGGCAATTTCGC	CGAATAACGGGACCAAAACC	20	20	50.0	50.0	58.9	57.1	2.9	0.0	1.811174	57086	57804	856-856	1.000000	65.6
psbK-trnQ-UUG_p4	psbK-trnQ-UUG	4	GCGTAGGCTTCAGGCAATTT	CGAATAACGGGACCAAAACC	20	20	50.0	50.0	59.2	57.1	2.9	0.0	2.094678	57086	57804	859-859	1.000000	63.3
psbK-trnQ-UUG_p5	psbK-trnQ-UUG	5	GTAGGCTTCAGGCAATTTCG	GAATAACGGGACCAAAACCC	20	20	50.0	50.0	57.2	56.7	2.9	0.0	2.142853	57086	57804	856-856	1.000000	63.0
ycf4_p1	ycf4	1	ATTTCCGGCAATTGCAATGG	TGGTTGTGCGTCTTGATCAA	20	20	45.0	45.0	58.0	58.0	30.3	8.1	0.066587	62582	63220	1499-1499	1.000000	80.1
ycf4_p2	ycf4	2	ATTTCCGGCAATTGCAATGG	TTGGTTGTGCGTCTTGATCA	20	20	45.0	45.0	58.0	58.0	30.3	0.0	0.066587	62582	63220	1500-1500	1.000000	80.1
ycf4_p3	ycf4	3	GAGGGCGAAATTCATCCAGT	TGGTTGTGCGTCTTGATCAA	20	20	50.0	45.0	58.0	58.0	4.8	8.1	0.083706	62582	63220	1920-1920	1.000000	79.8
ycf4_p4	ycf4	4	GAGGGCGAAATTCATCCAGT	TTGGTTGTGCGTCTTGATCA	20	20	50.0	45.0	58.0	58.0	4.8	0.0	0.083706	62582	63220	1921-1921	1.000000	79.8
ycf4_p5	ycf4	5	GGCAATTGCAATGGCTTCTT	TGGTTGTGCGTCTTGATCAA	20	20	45.0	45.0	57.9	58.0	32.4	8.1	0.143120	62582	63220	1493-1493	1.000000	79.4
ycf4-cemA_p1	ycf4-cemA	1	TCTAGGACTCCAGCCAAAGT	TAAAAGAGATGCACCAGGGC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.456096	63221	63554	488-488	1.000000	77.8
ycf4-cemA_p2	ycf4-cemA	2	AAAAGCTTGGGACTTGTCCA	TAAAAGAGATGCACCAGGGC	20	20	45.0	50.0	57.5	57.9	19.9	0.0	0.581371	63221	63554	462-462	1.000000	76.9
ycf4-cemA_p3	ycf4-cemA	3	TCTTGCGTGTACCAATGGAA	TAAAAGAGATGCACCAGGGC	20	20	45.0	50.0	57.4	57.9	6.2	0.0	0.681370	63221	63554	439-439	1.000000	76.2
ycf4-cemA_p4	ycf4-cemA	4	TTCTTGCGTGTACCAATGGA	TAAAAGAGATGCACCAGGGC	20	20	45.0	50.0	57.4	57.9	6.2	0.0	0.681370	63221	63554	440-440	1.000000	76.2
ycf4-cemA_p5	ycf4-cemA	5	TCTAGGACTCCAGCCAAAGT	GCACCAGGGCAAAAAGACTA	20	20	50.0	50.0	57.7	58.4	0.0	0.0	0.711167	63221	63554	478-478	1.000000	75.8
petA_p1	petA	1	AGGGAACTAATGCCAGTAACC	ATGTTGATAAATCCGCGTAC	21	20	47.6	40.0	57.4	53.9	0.0	4.9	5.731626	64456	65418	1048-1048	1.000000	40.1
petA_p2	petA	2	GGGAACTAATGCCAGTAACCT	ATGTTGATAAATCCGCGTAC	21	20	47.6	40.0	57.4	53.9	0.0	4.9	5.731626	64456	65418	1047-1047	1.000000	40.1
petA_p3	petA	3	GGGAACTAATGCCAGTAACC	ATGTTGATAAATCCGCGTAC	20	20	50.0	40.0	55.8	53.9	0.0	4.9	6.280448	64456	65418	1047-1047	1.000000	40.1
petA_p4	petA	4	AGCAGAATCATGGATAGGGAAC	ATGTTGATAAATCCGCGTAC	22	20	45.5	40.0	57.6	53.9	0.0	4.9	6.448882	64456	65418	1063-1063	1.000000	40.1
petA_p5	petA	5	GGGAACTAATGCCAGTAACCT	TATGTTGATAAATCCGCGTAC	21	21	47.6	38.1	57.4	53.9	0.0	4.9	6.699744	64456	65418	1048-1048	1.000000	40.1
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AACCGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.0	56.6	0.0	0.0	1.420951	67282	68803	1679-1680	1.000000	68.7
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	ACCGCTAGTAGAAAACCGAA	20	20	45.0	45.0	58.0	56.6	0.0	0.0	1.420951	67282	68803	1678-1679	1.000000	68.7
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AACCGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.9	56.6	6.4	0.0	1.454946	67282	68803	1668-1669	1.000000	68.5
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	ACCGCTAGTAGAAAACCGAA	20	20	50.0	45.0	57.9	56.6	6.4	0.0	1.454946	67282	68803	1667-1668	1.000000	68.5
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	ACCGCTAGTAGAAAACCGAA	20	20	50.0	45.0	57.8	56.6	24.9	0.0	1.559955	67282	68803	1590-1591	1.000000	67.6
rps3_p1	rps3	1	TCGAAAGATGGAACGTTAAAGA	AGCAGCAAATCCTAGTCACA	22	20	36.4	45.0	55.8	56.8	0.0	0.0	5.364719	86744	87397	810-810	1.000000	40.1
rps3_p2	rps3	2	TCGAAAGATGGAACGTTAAAGA	AGCAGCAAATCCTAGTCACAA	22	21	36.4	42.9	55.8	57.6	0.0	0.0	5.629592	86744	87397	810-810	1.000000	40.1
rps3_p3	rps3	3	TCGAAAGATGGAACGTTAAAGAT	AGCAGCAAATCCTAGTCACA	23	20	34.8	45.0	56.3	56.8	0.0	0.0	5.856783	86744	87397	810-810	1.000000	40.1
rps3_p4	rps3	4	CGAAAGATGGAACGTTAAAGA	AGCAGCAAATCCTAGTCACA	21	20	38.1	45.0	54.3	56.8	0.0	0.0	5.874173	86744	87397	809-809	1.000000	40.1
rps3_p5	rps3	5	TCGAAAGATGGAACGTTAAAG	AGCAGCAAATCCTAGTCACA	21	20	38.1	45.0	54.3	56.8	0.0	0.0	5.874173	86744	87397	810-810	1.000000	40.1
ycf1-rps15_p1	ycf1-rps15	1	ACCCATAATGGAAGCCGTTT	TCAACTGATCCGGTGTTTTCT	20	21	45.0	42.9	57.5	57.5	0.0	0.0	2.010568	118333	118823	605-625	1.000000	66.5
ycf1-rps15_p2	ycf1-rps15	2	ACCCATAATGGAAGCCGTTT	ACTGATCCGGTGTTTTCTTCT	20	21	45.0	42.9	57.5	57.2	0.0	0.0	2.296682	118333	118823	602-622	1.000000	64.2
ycf1-rps15_p3	ycf1-rps15	3	ACCCATAATGGAAGCCGTTT	TCAACTGATCCGGTGTTTTC	20	20	45.0	45.0	57.5	56.0	0.0	0.0	2.541096	118333	118823	605-625	1.000000	62.3
ycf1-rps15_p4	ycf1-rps15	4	ACCCATAATGGAAGCCGTTT	CAACTGATCCGGTGTTTTCT	20	20	45.0	45.0	57.5	56.0	0.0	0.0	2.551872	118333	118823	604-624	1.000000	62.2
ycf1-rps15_p5	ycf1-rps15	5	ACCCATAATGGAAGCCGTTT	ACTGATCCGGTGTTTTCTTCTT	20	22	45.0	40.9	57.5	57.9	0.0	0.0	2.611811	118333	118823	602-622	1.000000	61.7
rpl32-ndhF_p1	rpl32-ndhF	1	AAGGCTTTCAATGCTGTCCA	TGTTGCCGTGGGAAAAAGTA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.054584	129311	130079	963-963	1.000000	79.6
rpl32-ndhF_p2	rpl32-ndhF	2	AGGCTTTCAATGCTGTCCAA	TGTTGCCGTGGGAAAAAGTA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.054584	129311	130079	962-962	1.000000	79.6
rpl32-ndhF_p3	rpl32-ndhF	3	AAGGCTTTCAATGCTGTCCA	CGGGGTGAAAGGTATGATCC	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.256469	129311	130079	909-909	1.000000	78.0
rpl32-ndhF_p4	rpl32-ndhF	4	AGGCTTTCAATGCTGTCCAA	CGGGGTGAAAGGTATGATCC	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.256469	129311	130079	908-908	1.000000	78.0
rpl32-ndhF_p5	rpl32-ndhF	5	AGGCTTTCAATGCTGTCCAA	TTTGTTGCCGTGGGAAAAAG	20	20	45.0	45.0	58.0	57.7	0.0	0.0	0.301929	129311	130079	964-964	1.000000	77.7
ndhF_p1	ndhF	1	ACCGAACCCTTTACAAAAGAGA	GCGGATAACCCCAATTTAGGT	22	21	40.9	47.6	57.6	58.1	1.0	0.0	3.486577	130080	132314	2366-2366	1.000000	52.2
ndhF_p2	ndhF	2	ACCGAACCCTTTACAAAAGAGA	AGCGGATAACCCCAATTTAGG	22	21	40.9	47.6	57.6	57.8	1.0	0.0	3.650410	130080	132314	2367-2367	1.000000	50.9
ndhF_p3	ndhF	3	ACCGAACCCTTTACAAAAGAGA	TGAAGCTTTTCGGATGAGACA	22	21	40.9	42.9	57.6	57.6	1.0	0.0	3.827769	130080	132314	2415-2415	1.000000	49.4
ndhF_p4	ndhF	4	ACCGAACCCTTTACAAAAGAG	GCGGATAACCCCAATTTAGGT	21	21	42.9	47.6	56.1	58.1	1.0	0.0	3.964966	130080	132314	2366-2366	1.000000	48.3
ndhF_p5	ndhF	5	ACCGAACCCTTTACAAAAGAGAT	GCGGATAACCCCAATTTAGGT	23	21	39.1	47.6	58.0	58.1	1.0	0.0	4.078030	130080	132314	2366-2366	1.000000	47.4