Hotspot

rps18

Genome · 70483-70797 · 315 bp

Back to Wisteria

Pi 0.0381

MarkerSeek score 61.10

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: Genome
length_bp: 315
variable_sites: 12
pi: 0.0381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TTGTGTGTCACCCTTCCAAG	GAGATTTTCGCGGTTTGTGG	50.0	50.0	57.9	58.0	1046-1064 bp	1.0	81.7
rps18_p2	CTTGTGTGTCACCCTTCCAA	GAGATTTTCGCGGTTTGTGG	50.0	50.0	57.9	58.0	1047-1065 bp	1.0	81.6
rps18_p3	GGTTCATGGCCAAGGGTAAA	GAGATTTTCGCGGTTTGTGG	50.0	50.0	58.1	58.0	1266-1284 bp	1.0	81.2
rps18_p4	AAAGGAGGTTCATGGCCAAG	GAGATTTTCGCGGTTTGTGG	50.0	50.0	58.1	58.0	1272-1290 bp	1.0	81.2
rps18_p5	TGGAAATGGAATGCTAGGGC	GAGATTTTCGCGGTTTGTGG	50.0	50.0	57.9	58.0	1512-1530 bp	1.0	80.9