Hotspot

clpP-psbB

Genome · 73628-74716 · 1089 bp

Back to Wisteria

Pi 0.0347

MarkerSeek score 66.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: Genome
length_bp: 1089
variable_sites: 33
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GAACTCGAAACGGCACTTTG	ACCAAAACAAGCTACCCCTG	50.0	50.0	58.0	58.0	1443-1582 bp	1.0	85.8
clpP-psbB_p2	GAACTCGAAGTGCCATGCTA	ACCAAAACAAGCTACCCCTG	50.0	50.0	58.0	58.0	1879-1999 bp	1.0	85.7
clpP-psbB_p3	CACTGTTGACTTCTTGGCCT	ACCAAAACAAGCTACCCCTG	50.0	50.0	58.0	58.0	1530-1669 bp	1.0	85.6
clpP-psbB_p4	GAACTCGAAGTGCCATGCTA	ATCAAAACCTTCCACACCCC	50.0	50.0	58.0	58.0	2116 bp	0.5	59.8
clpP-psbB_p5	GAACTCGAAACGGCACTTTG	ATCAAAACCTTCCACACCCC	50.0	50.0	58.0	58.0	1699 bp	0.5	59.8