Hotspot

accD-psaI

Genome · 61236-61765 · 530 bp

Back to Wisteria

Pi 0.0227

MarkerSeek score 70.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: Genome
length_bp: 530
variable_sites: 12
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCATGCTTTCTTTCCTTTGCC	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.3	58.0	643-647 bp	1.0	70.9
accD-psaI_p2	TGCTTTCTTTCCTTTGCCTCA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.3	58.0	640-644 bp	1.0	70.7
accD-psaI_p3	CCATGCTTTCTTTCCTTTGCC	AAGAAGCCATTGCAATTGCC	47.6	45.0	58.3	57.9	649-653 bp	1.0	70.3
accD-psaI_p4	TGCTTTCTTTCCTTTGCCTCA	AAGAAGCCATTGCAATTGCC	42.9	45.0	58.3	57.9	646-650 bp	1.0	70.1
accD-psaI_p5	GCTTTCTTTCCTTTGCCTCA	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.5	58.0	639-643 bp	1.0	69.6