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trnQ-UUG-accD

Genome · 57417-59732 · 2316 bp

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Pi 0.0113

MarkerSeek score 53.30

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: Genome
length_bp: 2316
variable_sites: 25
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	CCGCCCTTACCACAAATGTA	55.0	50.0	58.1	57.9	2865-2910 bp	1.0	81.9
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	ATTTCCGCCCTTACCACAAA	55.0	45.0	58.1	57.7	2869-2914 bp	1.0	80.5
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	TTTCCGCCCTTACCACAAAT	55.0	45.0	58.1	57.7	2868-2913 bp	1.0	80.5
trnQ-UUG-accD_p4	GAGGTTCGAATCCTTCCGTC	ATTCCGGATGTTCCTGACAC	55.0	50.0	58.1	57.6	2521-2566 bp	1.0	80.0
trnQ-UUG-accD_p5	GAGGTTCGAATCCTTCCGTC	TTCCGCCCTTACCACAAATG	55.0	50.0	58.1	58.5	2867-2912 bp	1.0	79.2